Invitrogen™ CD196 (CCR6) Monoclonal Antibody (R6H1), PerCP-eFluor™ 710, eBioscience™

Mouse Monoclonal Antibody

Brand:  Invitrogen™ 46-1969-42

Product Code. 15539216

Description

Description: This R6H1 monoclonal antibody reacts with CD196 (also known as CCR6), a seven transmembrane G protein-coupled receptor expressed on T, B, dendritic, natural killer, and Langerhans cells. This CC chemokine receptor uniquely binds MIP-3a/CCL20, a chemoattractant for dendritic cells, effector/memory T cells, and B cells. CD196 is also involved in host defense and inflammation at epithelial sites. Furthermore, this receptor has been implicated in Th17 differentiation and CD4+FoxP3+ regulatory T cell development. Applications Reported: This R6H1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This R6H1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor...

CD196, also known as CCR6, is a member of the beta chemokine receptor family and is characterized by a seven transmembrane structure similar to G protein-coupled receptors. This receptor uniquely binds to macrophage inflammatory protein 3 alpha (MIP-3alpha/CCL20), which acts as a chemoattractant for dendritic cells, effector/memory T cells, and B cells. CCR6 is preferentially expressed by immature dendritic cells and memory T cells, playing a crucial role in host defense and inflammation at epithelial sites. It is important for B-lineage maturation and antigen-driven B-cell differentiation, and it regulates the migration and recruitment of dendritic and T cells during inflammatory and immunological responses. Additionally, CCR6 has been implicated in the differentiation of Th17 cells and the development of CD4+FoxP3+ regulatory T cells, highlighting its role in immune regulation. Alternatively spliced transcript variants encoding the same protein have been described for this gene, further emphasizing its functional diversity in immune processes.

Specifications

• Antigen: CD196 (CCR6)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P51684
• Gene Symbols: CCR6
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1235
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: R6H1
• Conjugate: PerCP-eFluor 710
• Gene: CCR6
• Gene Alias: BN-1; CC chemokine receptor 6; C-C chemokine receptor type 6; C-C CKR-6; C-C motif chemokine receptor 6; CC-CKR-6; CCR6; CCR-6; CD196; chemokine (C-C motif) receptor 6; chemokine (C-C) receptor 6; chemokine receptor-like 3; CKR6; CKRL3; CKR-L3; Cmkbr6; DCR2; DRY6; DRY-6; G protein-coupled receptor 29; GPR29; GPRCY4; GPR-CY4; G-protein coupled receptor 29; KY411; LARC receptor; seven-transmembrane receptor, lymphocyte, 22; STRL22
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ