CD14 Monoclonal Antibody (MEM-15), NovaFluor™ Red 685, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
17276546	Invitrogen H007T02R02	25 Tests
17226566	Invitrogen H007T03R02	100 Tests

Description

The MEM-15 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.

Applications Reported: This MEM-15 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MEM-15 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 4 μL (0.6 μg) per test. A test is defined as the amount ( μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.

When using more than one NovaFluor dye, CellBlox Monocyte and Macrophage Blocking Buffer may be used to block non-specific binding of dyes to monocytes.

Excitation: 637 nm; Emission: 685 nm; Laser: 633-640 nm (Red) Laser

CD14 Monoclonal antibody specifically detects CD14 in Human, Non-human Primate samples. It is validated for Flow Cytometry

Specifications

• Antigen: CD14
• Concentration: 4 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Gene Alias: CD 14; CD14; CD14 antigen; CD14 molecule; cd14 monocyte; lipopolysaccharide receptor; lipoprotein receptor; LPSR antibody; Monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form; Monocyte differentiation antigen CD14, urinary form; Myeloid cell-specific leucine-rich glycoprotein; myeloid membrane glycoprotein precursor; sCD14; soluble CD14
• Isotype: IgG1
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: MEM-15
• Applications: Flow Cytometry
• Conjugate: NovaFluor Red 685
• Host Species: Mouse
• Target Species: Human, Non-human Primate
• Gene Accession No.: P08571
• Gene ID (Entrez): 929
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: Cd14