CD14 Monoclonal Antibody (MEM-15), NovaFluor™ Red 685, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen H007T02R02

Product Code. 17276546

Description

The MEM-15 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.

Applications Reported: This MEM-15 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MEM-15 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 4 μL (0.6 μg) per test. A test is defined as the amount ( μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.

When using more than one NovaFluor dye, CellBlox Monocyte and Macrophage Blocking Buffer may be used to block non-specific binding of dyes to monocytes.

Excitation: 637 nm; Emission: 685 nm; Laser: 633-640 nm (Red) Laser

CD14 Monoclonal antibody specifically detects CD14 in Human, Non-human Primate samples. It is validated for Flow Cytometry

Specifications

• Antigen: CD14
• Classification: Monoclonal
• Concentration: 4 μL/Test
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P08571
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human, Non-human Primate
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry
• Clone: MEM-15
• Conjugate: NovaFluor Red 685
• Gene: Cd14
• Gene Alias: CD 14; CD14; CD14 antigen; CD14 molecule; cd14 monocyte; lipopolysaccharide receptor; lipoprotein receptor; LPSR antibody; Monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form; Monocyte differentiation antigen CD14, urinary form; Myeloid cell-specific leucine-rich glycoprotein; myeloid membrane glycoprotein precursor; sCD14; soluble CD14
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 929
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid