missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protein Purification Reagents and Kits
  6. Thermo Scientific™ CaptureSelect™ tPA Affinity Matrix

Thermo Scientific™ CaptureSelect™ tPA Affinity Matrix

Product Code. 18426608 Shop All Thermo Scientific Products
Change view
Click to view available options
Quantity:
5 mL
10 mL
50 mL
Unit Size:
10mL
50mL
5mL
3 product options available for selection
Product selection table with 3 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
18426608 50 mL 50mL
18426408 5 mL 5mL
18476658 10 mL 10mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 3 options available.
3 options
This item is not returnable. View return policy
Product Code. 18426608 Supplier Thermo Scientific™ Supplier No. 2943430050

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Thermo Scientific™ CaptureSelect™ tPA Affinity Matrix has been specifically designed for the purification of recombinant human tissue plasminogen activator (tPA).

CaptureSelect tPA Affinity Matrix has been specifically designed for the purification of recombinant human tissue plasminogen activator (tPA). This matrix can be used for the purification of recombinant human tPA from cell culture harvests with high purity and yield in a single step. The design of the tPA affinity matrix allows elution of the tPA protein at a neutral pH in the presence of magnesium chloride, but standard low-pH elution buffers like 0.1 M glycine, pH 3.0, can also be used.

Features of this affinity matrix include:

  • Neutral pH elution with 20 mM Tris, 2.0 M MgCl2, 0.2 M arginine pH 7.0
  • Caustic cleaning with 50 mM NaOH
  • Excellent scalability
  • Non-animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

  • Matrix: agarose-based, epoxide-activated
  • Average particle size: 65 ± 10 μm
  • Ligand: CaptureSelect tPA affinity ligand
  • Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
  • Binding capacity: 8-10 g/L depending on flow rate, column height, and contact time
  • Elution conditions: Neutral pH: 20 mM Tris, 2.0 M MgCl2, 0.2 M arginine pH 7.0; Low pH: 0.1 M glycine, pH 3.0
  • Flow characteristics: 50–200 cm/h (up to 2 bar)
  • Formulation buffer: 20% (v/v) ethanol

Specifications

Binding Capacity 8 to 10 g/L
Flow Rate 50 to 200 cm/hr. (Up to 2 bar)
Matrix Agarose-based, epoxide-activated
Content And Storage Store in refrigerator (2°C to 8°C).
Particle Size 65 ±10 μm
Form Agarose Bead
Product Line CaptureSelect
Quantity 50 mL
Type Affinity Matrix
Ligand Type TPA affinity ligand
Stationary Phase Anti-tPA
Show More Show Less
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.