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  6. Thermo Scientific™ CaptureSelect™ AAT-XL Affinity Matrix

Thermo Scientific™ CaptureSelect™ AAT-XL Affinity Matrix

Product Code. 18406808 Shop All Thermo Scientific Products
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Product Code. 18406808 Supplier Thermo Scientific™ Supplier No. 2943832010

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Description

Thermo Scientific™ CaptureSelect™ AAT-XL Affinity Matrix has been specifically designed for the purification of native human alpha-1 antitrypsin (AAT) from complex sources like plasma/serum and recombinant human AAT from cell culture supernatants.

CaptureSelect AAT-XL Affinity Matrix has been specifically designed for the purification of native human alpha-1 antitrypsin (AAT) from complex sources like plasma/serum and recombinant human AAT from cell culture supernatants. This affinity matrix offers mild elution conditions at pH 6. The high selectivity and yield obtained using CaptureSelect AAT-XL Affinity Matrix enable a robust and efficient purification process with excellent purity obtained in a single step.

Features of this affinity matrix include:

  • Highly selective for native and recombinant human AAT
  • High recovery and yield obtained in a single purification step
  • Mild elution at pH 6
  • Excellent scalability
  • Non-animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

  • Matrix: agarose-based, epoxide-activated
  • Average particle size: 65 ± 10 μm
  • Ligand: CaptureSelect AAT-XL affinity ligand
  • Ligand coupling method: epoxide coupling
  • Binding capacity: 15 mg AAT/mL resin
  • Elution conditions: 20 mM Tris, 2.0 M MgCl2, pH 6.0
  • Flow characteristics: 50–200 cm/h (up to 2 bar)
  • Formulation buffer: 20% (v/v) ethanol

Specifications

Binding Capacity 15 mg AAT/mL resin
Flow Rate 50 to 200 cm/hr. (Up to 2 bar)
Matrix Agarose-based, epoxide-activated
Particle Size 65 ±10 μm
Form Resin Slurry
Product Line CaptureSelect
Quantity 10 mL
Type Affinity Matrix
Ligand Type AAT-XL affinity ligand
Product Title
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