Invitrogen™ B7-H7 Monoclonal Antibody (MA57YW), PE-Cyanine7, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen™ 25-6537-42

Product Code. 16390784

Description

Description: This MA57YW monoclonal antibody recognizes human B7-H7 on live and paraformaldehyde-fixed cells. Applications Reported: This MA57YW antibody has been reported for use in flow cytometric analysis. This MA57YW monoclonal antibody recognizes human B7-H7 on live and paraformaldehyde-fixed cells. Applications Tested: This MA57YW antibody has been pre-diluted and tested by flow cytometric analysis of the HDLM-2 cell line. This may be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

B7-H7 (also known as HHLA2) is a membrane protein with three Ig-like domains (IgV-IgC-IgV), whereas other members of the B7 family generally have only two Ig domains (IgV-IgC). B7-H7 is somewhat more closely related to B7-H3 and B7-H4 and shares 10% to 18% amino acid identity and 23% to 33% similarity to B7 family members. B7-H7 protein is widely expressed in epithelium cells of the gallbladder, breast, kidney, colon, small intestines and placenta. In the immune system, HHLA2 protein is not constitutively expressed on human monocytes/macrophages. B7-H7 is widely expressed in cancers, especially in triple negative breast cancer.

Specifications

• Antigen: B7-H7
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9UM44
• Gene Symbols: HHLA2
• Immunogen: B7-H7 recombinant protein.
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: MA57YW
• Conjugate: PE-Cyanine7
• Gene: HHLA2
• Gene Alias: B7-H5; B7H7; B7-H7; B7y; HERV-H LTR-associating 2; HERV-H LTR-associating protein 2; HHLA2; Human endogenous retrovirus-H long terminal repeat-associating protein 2
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 11148
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid