Axl Monoclonal Antibody (MAXL8DS), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15913314	eBioscience 53108480	25 μg

Description

Description: This MAXL8DS monoclonal antibody recognizes mouse Axl, a member of the TAM family of tyrosine kinase receptors that also includes MerTK and Tyro3. Within hematopoietic compartment the highest expression of Axl can be observed on dendritic cells and macrophages. Stimulation with TLR ligands has been shown to additionally upregulate this expression. This MAXL8DS antibody will work in flow cytometry on both native and fixed/permeabilized cells. Applications Reported: This MAXL8DS antibody has been reported for use in flow cytometric analysis. Applications Tested: This MAXL8DS antibody has been tested by flow cytometric analysis of mouse bone marrow cells cultured with GM-CSF. This may be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma. The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.

Specifications

• Antigen: Axl
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: Q00993
• Immunogen: C57BL/6 mouse Axl-human IgG Fc fusion protein
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

• Clone: MAXL8DS
• Applications: Flow Cytometry
• Conjugate: Alexa Fluor 488
• Host Species: Rat
• Target Species: Mouse
• Gene ID (Entrez): 26362
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody