Axl Monoclonal Antibody (MAXL8DS), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  eBioscience 53108480

Product Code. 15913314

Description

Description: This MAXL8DS monoclonal antibody recognizes mouse Axl, a member of the TAM family of tyrosine kinase receptors that also includes MerTK and Tyro3. Within hematopoietic compartment the highest expression of Axl can be observed on dendritic cells and macrophages. Stimulation with TLR ligands has been shown to additionally upregulate this expression. This MAXL8DS antibody will work in flow cytometry on both native and fixed/permeabilized cells. Applications Reported: This MAXL8DS antibody has been reported for use in flow cytometric analysis. Applications Tested: This MAXL8DS antibody has been tested by flow cytometric analysis of mouse bone marrow cells cultured with GM-CSF. This may be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

Axl is a member of the TAM receptor family, which also includes also MerTK and TYRO3. Axl contains protein kinase activity and is activated by dimerization and auto-phosphorylation upon ligand binding. In response to its ligands, Protein S and GAS6, Axl activates anti-inflammatory pathways (through SOCS-1 and SOCS-3), which limits TLR and cytokine signaling. This results in dampened inflammatory responses in macrophages and DCs. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Soluble Axl can serve as marker of liver fibrosis, cirrhosis and a number of maligcies.

Specifications

• Antigen: Axl
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q00993
• Gene Symbols: Axl
• Immunogen: C57BL/6 mouse Axl-human IgG Fc fusion protein
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: MAXL8DS
• Conjugate: Alexa Fluor 488
• Gene: Axl
• Gene Alias: ARK, AZF1, UFO, Tyro7
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 26362
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid