Caspase-8 Mouse anti-Human, Unlabeled, Clone: 3-1-9, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 551242

Product Code. 15816588

Description

Caspase-8 (FLICE/MACH-1) is a 55kDa cytosolic protein with homology to the CD95/Fas-associated signal transducer, FADD/MORT-1, as well as to other caspase (ICE/Ced-3) cysteine proteases. The N-terminal region of caspase-8 contains an amino acid sequence, termed the death domain, that facilitates caspase-8-FADD direct interaction. FADD therefore acts as an adapter molecule, allowing caspase-8 to become recruited to the cytoplasmic region of Fas following receptor activation. Viral proteins (v-FLIPS) which inhibit recruitment and activation of
caspase-8 have been isolated. Caspase-8 is produced as a proenzyme (55/50kDa doublet) which upon receptor aggregation is proteolytically cleaved into smaller subunits of 40/36 (doublet), and 23kDa. Overexpression of caspase-8 is sufficient to induce apoptosis in certain cell lines (e.g., MCF-7) and this phenotype is blocked by overexpression of the caspase-3 protease inhibitor, CrmA. The antibody recognizes both the proform of human caspase-8 (55/50kDa doublet) as well as the cleaved forms which migrate at 40/36 (doublet) and 23kDa in SDS/PAGE. It
also immunoprecipitates the full-length human caspase-8. Full-length recombinant human caspase-8 protein was used as immunogen.

Host Species: Mouse
Clone: 3-1-9
Isotype: IgG1 κ
Species Reactivity: Human
Immunogen: Human Caspase-8 full-length recombinant protein

Immunoprecipitation, Western Blotting

Specifications

• Antigen: Caspase-8
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Description: FLICE, MACH-1, Mch5
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

• Applications: Western Blot
• Clone: 3/1/2009
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Human Caspase-8 full-length recombinant protein
• Quantity: 50 μg
• Research Discipline: Cell Biology
• Target Species: Human
• Isotype: IgG1 κ

For Research Use Only.