Thermo Scientific™ AminoLink™ Immobilization Kit

Product Code. 10259883

Brand: Thermo Scientific™ 44890

Description

Covalently immobilize proteins and antibodies by lysine residues or other primary amines (-NH2) via reductive amination with these aldehyde-activated agarose beads.

Thermo Scientific™ AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilization of antibodies and other proteins through primary amines.

AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups. Proteins and other molecules with primary amines can be covalently attached (immobilized) to the resin to make chromatography columns for use in affinity purification. The aldehyde groups form stable secondary amine bonds with primary amines such as exist in the side chain of lysine (K) residues, which are generally abundant and readily accessible in proteins. Once a protein is immobilized, the prepared affinity resin can be used for a variety of batch and column affinity purification methods involving binding interactions with the immobilized protein. The resin and linkage are stable in most binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for multiple rounds of affinity purification procedures.

Highlights:

• AminoLink Coupling Resin – aldehyde-activated crosslinked 4% beaded agarose

• Ideal for antibodies and other proteins – immobilize molecules via primary amines (-NH₂)

• Flexible coupling conditions – efficient (>85%) coupling over a wide range of pH (4 to 10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent

• Stable, permanent immobilization – coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand

• Better than immobilization to CNBr-activated agarose – bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures

• Versatile and reusable – prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions

Includes:

Kit contains pre-packed AminoLink Columns, coupling buffer, quenching buffer, wash solution, sodium cyanoborohydride solution, and column accessories

Specifications

• Description: AminoLink Immobilization Kit, 2mL
• Content And Storage: Sufficient For: Immobilizing 2 to 20 mg of protein per column
  • AminoLink Columns, 2mL, 5 columns
  • AminoLink Coupling Buffer (pH 7.0), 250 mL
  • Quenching Buffer, 50 mL
  • Wash Buffer, 240 mL
  • Sodium Cyanoborohydride Solution, 5M, 0.5 mL
  • Column Accessories
  
  Store at 4°C
• Product Line: AminoLink
• Quantity: 5 Column Kit
• Target: Antibody
• Type: Immobilization Kit
• Stationary Phase: Aldehyde
• Sufficient For: 5 Protein Immobilizations
• Format: Spin Column
• Column Type: Affinity, Agarose Resin

Safety and Handling

Product Identifier

• AminoLink Immobilization Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 2
• ACUTE AQUATIC Acute 1
• Serious eye damage/eye irritation Category 1
• Skin corrosion/irritation Category 1 B

Hazard Statement

• H300-Fatal if swallowed.
• H310-Fatal in contact with skin.
• H314-Causes severe skin burns and eye damage.
• H318-Causes serious eye damage.
• H330-Fatal if inhaled.
• H400-Very toxic to aquatic life.
• EUH032-Contact with acids liberates very toxic gas.
• EUH204-Contains isocyanates. May produce an allergic reaction.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P262-Do not get in eyes, on skin, or on clothing.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P331-Do NOT induce vomiting.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Sodium hydroxide

For Research Use Only. Not for use in diagnostic procedures.