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Description
Cy3 and Cy5 mono-functional maleimides are particularly suitable for the selective labeling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides, and oligonucleotides. They are also useful in antibody labeling since the dye molecules are introduced away from the antibody binding site, increasing the chance that functionality of the labeled molecule will be unimpaired. The protocol is simple and robust, offering an alternative chemistry when either greater selectivity of labeling is required or amine labeling with Cy3 and Cy5 NHS esters is not desirable or appropriate.
- Provides an alternative for when CyDye™ NHS esters are unsuitable.
- Dyes are packaged in premeasured amounts and foil-sealed to ensure consistent labelings
Order Info
Each kit contains five vials of dye, each sufficient for labeling 1mg of protein.
Specifications
Specifications
| For Use With (Application) | For the labelling of biological compounds with CyTM5 monofunctional dye |
| Quantity | 5 vials each sufficient to label 1 mg protein |
| Product Type | Cy5 Maleimide Mono-Reactive Dye 5-Pack |
| Kit Contents | Five vials of dye |
| Sufficient For | Labeling 1mg Protein |
| Molecular Weight (g/mol) | 817 |
| Solubility | Soluble in cold water and hot water |
| Content And Storage | 2°C to 8°C, protected from light. Reconstituted material may be stored for up to 2 weeks at -20°C in aliquots to avoid repeat freeze thaw cycles. |
| Label or Dye | Cy5 |
| Color | Blue |
Safety and Handling
- Danger
- Acute toxicity Category 3
- Skin sensitiser Category 1
- Respiratory sensitiser Category 1
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