Molecular Probes™ Alexa Fluor™ 647 Microscale Protein Labeling Kit

Product Code. 10750184

Brand: Molecular Probes™ A30009

Description

Microscale Protein Labeling Kits provide a convenient means for attaching a fluorescent label to a small amount of antibody or protein (20–100 μg).

Microscale Protein Labeling Kits provide a convenient means for attaching a fluorescent label to a small amount of antibody or protein (20–100 μg). The kits are available in four Alexa Fluor™ colors (or biotin) and supply everything needed for three labeling and separation reactions.

Important Features of Microscale Protein Labeling Kits:

• Labeled proteins typically ready to use typically in 2 hours (∼30 minutes hands-on time)
• Optimized for 20–100μg of protein with molecular weights between 12 and 150 kDa
• Purified using convenient spin filters with yields between 60 and 90%
• Stabilizing proteins must be removed from the sample before labeling

Stable Reaction Chemistry and Superior Alexa Fluor™ Dyes

In the Microscale Protein Labeling Kits, the reactive dye contains a succinimidyl (NHS) ester moiety that reacts with primary amines of proteins to form stable dye-protein conjugates. Compared to traditional dyes, Alexa Fluor™ dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. And generally when using Alexa Fluor™ dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor™ Dyes Spanning the Visible and Infrared Spectrum—Section 1.3.

Learn More About Protein and Antibody Labeling

We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See Antibody Labeling from A to Z or use our Labeling Chemistry Selection Tool for other choices. To learn more about our various kits read Kits for Labeling Proteins and Nucleic Acids—Section 1.2 in the Molecular Probes™ Handbook.

We'll Make a Custom Antibody Conjugate for You

If you can't find what you're looking for in our stocked list, we'll prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.

Specifications

• Product Type: Labeling Kit
• Quantity: 1 Kit
• Content And Storage: Store in refrigerator 2°C to 8°C and protect from light.
• Detection Method: Fluorescence
• Excitation/Emission: 650/665 nm
• Labeling Method: Conjugation-based
• Labeling Scale: 20–100 μg
• Shipping Condition: Room Temperature
• Product Line: Alexa Fluor™
• Reactive Moiety: Succinimidyl (NHS) Ester
• Label or Dye: Alexa Fluor 647
• Color: Far-Red
• Labeling Target: Proteins
• Label Type: Alexa Fluor

For Research Use Only. Not for use in diagnostic procedures.