Thermo Scientific™ ActivX™ Fluorophosphonate Probes
Tagged phosphonate probes to assay serine hydrolase active sites using fluorescence, biotin-affinity purification or detection, or mass spectrometry.
Brand: Thermo Scientific™ 88317
Code : PN
Additional Details : Weight : 0.01000kg
DescriptionActivX FP Probes feature a reactive fluorophosphonate group that specifically and covalently labels the active-site serine of enzymatically active serine hydrolases. These probes are available with a desthiobiotin (biotin analog) tag for selective enrichment, TAMRA fluorophore for detection or a reactive- azido group (Staudinger reagents) that facilitates multiplex labeling when used with phosphine- or alkyne-derivatized tags. These probes can be used to assess activity or screen small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins.
Specific – labels the reactive site of active serine hydrolases
Compatible – tags available for capture, detection and Staudinger conjugation
Flexible – use for in vitro or intracellular enzyme labeling
Determine serine hydrolase enzyme activity in cells and lysates; Mapping the active-site serine of functionally diverse serine hydrolase family members (e.g. proteases, lipases, esterases); Screen for small molecule binding affinities and active-site inhibition; Profile serine hydrolases using fluorescent, Western blot or mass spectrometry workflows
|ActivX Desthiobiotin-FP Serine Hydrolase Probe|
|Desthiobiotin-fluorophosphonate, MW 464|
|100 labeling reactions or 10 pull-down assays|