Thermo Scientific™ ActivX™ Fluorophosphonate Probes

Tagged phosphonate probes to assay serine hydrolase active sites using fluorescence, biotin-affinity purification or detection, or mass spectrometry.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 88317

4.6 UG ACTIVX DESTHIOBIOTIN-FP SERINE HYDROLASEprobe sufficient for100 labelling reactions of 10

UNSPSC: 41106510

Code: PN

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 11844624

Quantity Price
1 £ 334.0 / Each
EU Stock 7
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Description and Specification

Specification

Description ActivX Desthiobiotin-FP Serine Hydrolase Probe
Formulation Desthiobiotin-fluorophosphonate, MW 464
Quantity 4.6µg
Sufficient For 100 labeling reactions or 10 pull-down assays

ActivX FP Probes feature a reactive fluorophosphonate group that specifically and covalently labels the active-site serine of enzymatically active serine hydrolases. These probes are available with a desthiobiotin (biotin analog) tag for selective enrichment, TAMRA fluorophore for detection or a reactive- azido group (Staudinger reagents) that facilitates multiplex labeling when used with phosphine- or alkyne-derivatized tags. These probes can be used to assess activity or screen small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins.

Highlights:

  • Specific – labels the reactive site of active serine hydrolases

  • Compatible – tags available for capture, detection and Staudinger conjugation

  • Flexible – use for in vitro or intracellular enzyme labeling

Recommended for:

Determine serine hydrolase enzyme activity in cells and lysates; Mapping the active-site serine of functionally diverse serine hydrolase family members (e.g. proteases, lipases, esterases); Screen for small molecule binding affinities and active-site inhibition; Profile serine hydrolases using fluorescent, Western blot or mass spectrometry workflows