Thermo Scientific™ ActivX™ Fluorophosphonate Probes

Tagged phosphonate probes to assay serine hydrolase active sites using fluorescence, biotin-affinity purification or detection, or mass spectrometry.

Brand: Thermo Scientific™

Manufacturer Part Number: 88316

UNSPSC: 41106510

Code: PN

Additional Details:
Additional Details: Weight: 1.36080kg

Product Code. 11834624

Quantity Price
1 £ 334.0 / Each
Estimated Shipment date
from Supplier 12-05-2017
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Description and Specification


Formulation Azido-fluorophosphonate, MW 350
Sufficient For 100 labeling reactions or 10 pull-down assays
Description ActivX Azido-FP Serine Hydrolase Probe
Quantity 3.5µg

ActivX FP Probes feature a reactive fluorophosphonate group that specifically and covalently labels the active-site serine of enzymatically active serine hydrolases. These probes are available with a desthiobiotin (biotin analog) tag for selective enrichment, TAMRA fluorophore for detection or a reactive- azido group (Staudinger reagents) that facilitates multiplex labeling when used with phosphine- or alkyne-derivatized tags. These probes can be used to assess activity or screen small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins.


  • Specific – labels the reactive site of active serine hydrolases

  • Compatible – tags available for capture, detection and Staudinger conjugation

  • Flexible – use for in vitro or intracellular enzyme labeling

Recommended for:

Determine serine hydrolase enzyme activity in cells and lysates; Mapping the active-site serine of functionally diverse serine hydrolase family members (e.g. proteases, lipases, esterases); Screen for small molecule binding affinities and active-site inhibition; Profile serine hydrolases using fluorescent, Western blot or mass spectrometry workflows