Molecular Probes™ 6-NBDG (6-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-6-Deoxyglucose)

Product Code.	Quantity	unitSize
11569126	5 mg	5mg

Product Code. 11569126 Supplier Molecular Probes™ Supplier No. N23106

Description

6-NBDG (6-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-6-Deoxyglucose)

6-NBDG is a fluorescent nonhydrolyzable glucose analog that has been used to monitor glucose uptake and transport in live cells. Although sensitive to its environment NBD fluorescence typically displays excitation/emission maxima of ∼465/540 nm and can be visualized using optical filters designed for fluorescein.

Specifications

• Color: Orange
• Content And Storage: Store in freezer at -5°C to -30°C and protect from light.
• Description: 6-NBDG (6-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-6-Deoxyglucose)
• Detection Method: Absorbance
• Form: Solid
• Product Line: Life Technologies
• Quantity: 5 mg
• Shipping Condition: Room Temperature

Frequently Asked Questions (FAQs)

What are the final concentrations of 2-NBDG and 6-NBDG, and incubation times that may be used?

Final concentrations of 2-NBDG can range from 10 µM to 600 µM; 100 µM to 600 µM have been used with bacteria and yeasts and, from 10 µM to 200 µM for primary and cultured mammalian cells.

Final concentrations of 6-NBDG may range from 30 µM up to 300 µM.

Cells should be incubated at the desired temperature and time to allow for sufficient detection of green-yellow fluorescence within cells. Uptake is temperature dependent. Incubation times may range from seconds up to 30 minutes or longer, dependent upon the final concentration of the reagent, cell type, culture conditions, and other factors.

What solvents can be used to resolubilize either 2-NBDG or 6-NBDG to make a stock solution, and how should this stock solution be stored?

Compatible solvents (and approximate maximum solubility): DMSO ( ˜10 mg/mL), DMF (˜10 mg/mL), ethanol (˜20 mg/mL), methanol (˜20 mg/mL), H₂O (˜10 mg/mL), PBS, pH 7.2 (˜10 mg/mL).

Stock solutions may be prepared using the recommended solvents in concentrations at or below their maximum solubility and then stored frozen, desiccated (for non-aqueous solvents), and protected from light. Aqueous solutions should not be stored longer than one day.

For long-term storage, these reagents should be stored as a solid at ≤–20°C. To make smaller aliquots, dissolve the reagent in ethanol, make smaller aliquots in separate vials and then evaporate the solvent using a vacuum pump. Do not use DMSO or DMF due to their low vapor pressure.

For Research Use Only. Not for use in diagnostic procedures.