Thermo Scientific™ 5-IAF (5-iodoacetamido-fluorescein)

Product Code. 10649148

Brand: Thermo Scientific™ 62246

Description

5-IAF (5-iodoacetamido-fluorescein)

Thermo Scientific 5-Iodoacetamido-Fluorescein (5-IAF) is a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes. 5-Iodoacetamido-Fluorescein is a sulfhydryl-reactive derivative of fluorescein that labels proteins and other molecules having free thiols (cysteine side chains)

Properties of 5-Iodoacetamido-Fluorescein:

• Alternative names: 5-IAF, 5-iodoacetamidofluorescein
• Chemical name: Acetamide, N-(3',6'-dihydroxy-3-oxospiro (isobenzofuran-1(3H), 9'-(9H)xanthen)-5-yl)-2-iodo
• Molecular weight: 515.26±3
• Excitation source: 488 nm spectral line, argon-ion laser
• Excitation wavelength: 494 nm
• Emission wavelength: 518 nm
• Extinction coefficient: > 80,000 M^-1cm^-1
• CAS #: 63368-54-7
• Solubility: Soluble in DMF; aqueous buffers at pH > 6
• Reactive groups: Iodoacetamide, reacts with sulfhydryls at pH 7.0 to 7.5
  
  Applications:
• Label antibodies for use as immunofluorescent probes
• Label oligonucleotides for hybridization probes
• Detect proteins in gels and on Western blots

Specifications

• Description: 5-IAF (5-iodoacetamido-fluorescein), 25mg, Yellow to Orange, Powder, FITC Dye, 515.26±3 Molecular Weight, 494/518nm Excitation/Emission Wavelength, 63368-54-7 CAS Number, –20°C Storage Temperature, Used for Protein Biology, Protein Labeli
• Format: Powder
• For Use With (Application): Protein labeling and crosslinking, protein biology
• Quantity: 25 mg
• Product Type: 5-IAF (5-iodoacetamido-fluorescein)
• Molecular Weight (g/mol): 515.26±3
• Purity: >99% by HPLC
• Content And Storage: -20°C
• Label or Dye: FITC (Fluorescein)
• Color: Yellow to Orange
• Emission: 518
• Excitation: 494

For Research Use Only. Not for use in diagnostic procedures.