DiscoverX PathHunter™ eXpress CCKAR Activated GPCR Internalization Assay

Codice prodotto.	Quantity	unitSize
17303599	1,000 dp (10 x 96-well)	1 set

Codice prodotto. 17303599 Fornitore DiscoverX N. del fornitore 930650E3CP5L

Descrizione

The PathHunter™ eXpress CCKAR Activated GPCR Internalization Assay measures CCKAR (GPCR) internalization via early-endosome localization. eXpress kits contain all assay materials: cells, reagents, and plates.

The PathHunter™ CCKAR Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit assesses ligand (e.g. cholecystokinin ) based internalization of CCKAR via detection of localization to the early endosome. This product uses EFC technology. The included cells overexpress EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged CCKAR. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting CCKAR-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Specifica

• Assay Entry: GPCR
• For Use With (Application): Assay-Ready eXpress Kits
• Quantity: 1,000 dp (10 x 96-well)
• Target Entry: CCKAR
• Cell Line: U2OS
• Target: CCKAR