Whatman Filtration for HPLC Sample Preparation

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Simplify sample preparation with the Whatman regenerated cellulose membrane portfolio from Cytiva

Please find in the training section, interesting papers relating to analytical HPLC from Cytiva

Overview

Accurate. Reliable. Fast

  • Reduce cost with well-designed devices – the regenerated cellulose (RC) family includes products for robotic sample handing, outside-of-lab sample preparation, certified method development, and working with hard-to-filter samples.
  • Increase accuracy with reliable, repeatable results – The RC family is demonstrated to have extremely low levels of chemical extractables that could interfere with analysis. Products are available to retain a variety of particle sizes.
  • Save time with multifunctional products – reduce time spent deciding which membrane type to use for your sample preparation. The RC membrane is broadly compatible with common HPLC sample solvents and mobile phases.
  • Improve efficiency with ready-to-use products – RC products are supplied ready for use. The hydrophilic, low-protein binding RC membrane is suitable for a wide variety of HPLC applications.

Characteristics of common membranes

Solvent compatibility

Qualities

Membrane1

Aqueous

Nonaqueous

Low protein binding

Low extractables

RC++++++++++++
CA+++-++++
ME+++++-
CN+++---
PVDF++++++++++
PP++++++--
NYL++++--
PES++++++++++
PTFE-+++-+
Anopore™++++++++++
PC++++++++++
1 RC = regenerated cellulose; CA = cellulose acetate; ME = mixed ester; CN = cellulose nitrate; PVDF = polyvinylidene fluoride; PP = polypropylene; NYL = nylon; PES = polyethersulfone; PTFE = Polytetrafluoroethylene; PC = polycarbonate.
+++ strong characteristic
+ weak characteristic
- does not have characteristi
RC Membrane

Mobile phase filtration

Membrane_filter_Regenerated_Cellulose_RC55

Whatman RC55 and GV 050/2 vacuum filtration unit for solvent filtration

Use the same material for mobile phase filtration and sample filtration to:

  • Reduce variation of analysis
  • Reduce rate of column clogging
  • Enhance lifetime of column
If inline degassing is required, consider the Whatman Inline filter/de-gasser. Choose from two membrane options:
  • Nylon – when mobile phase is > 20% aqueous
  • Polypropylene – for non-aqueous solvents

RC Membrane

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Puradisc Filters

Preventive care: Puradisc filters

Filtration of your samples is important as a preventive maintenance step for HPLC or UHPLC analysis. Keep unwanted particulate matter from entering the injector to increase column life, shorten run time, and optimize peak shape.

  • Manufactured to a high standard in Cytiva’s ISO 9001:2015 certified facilities
  • Available in non-RC membrane types to support a variety of other applications
Puradisc_Filters

Typical data

Puradic Syringe Filters

 

4 mm diameter*

30 mm diameter*

HousingPolypropylenePolycarbonate
Filtration area0.2 cm25.7 cm2
Maximum pressure75 psi (5.2 bar)100 psi (6.9 bar)
Volume ‘hold up’ with air purge< 10 μl< 50 μl
Dimensions10.1 × 23.5 mm
19.5 × 7.7 mm
(PVDF membrane only)
26 × 34 mm
Weight (approx)0.55 g4.7 g
Volume throughputup to 2 mlup to 100 ml
Inlet connectionFemale Luer lockFemale Luer lock
Outlet connectionMale Luer/tube tipMale Luer/tube tip
Sterilization§Autoclave at 121°CAutoclaving not recommended
*Diameters available for Puradisc only.
§Applies to nonsterile filters only. Do not autoclave sterile filters

30mm Puradisc Syringe Filters

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4mm Puradisc Syringe Filters

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Spartan Filters

Certified quality for method development: SPARTAN filters

Spartan_Filters

SPARTAN brand syringe filters are HPLC-certified for confidence and consistent results. Tested and certified for the absence of UV-absorbing substances (210 and 254 nm) with water, methanol, acetonitrile to ensure absence of interfering substances.

  • Hydrophilic, low protein-binding membrane made of regenerated cellulose
  • Excellent chemical resistance against the standard aqueous and organic HPLC solvents
  • Tested and certified for the absence of UV-absorbing substances at wavelengths of 210 and 254 nm with water, methanol, and acetonitrile
  • 13 mm diameter with Mini-Tip options
  • 13 mm diameter with extremely low dead volume < 10 μL

Spartan filters 13mm diameter

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Spartan filters 30mm diameter

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Whatman GDX

High-particulate, challenging sample filtration: Whatman GD/X filters

Whatman_GDX_Syringe_Filters

Filter even the most difficult samples and use less hand pressure with GD/X syringe filters.

  • Exceptional loading capacity and fast flow rates – prevents back pressure and clogging of membrane
  • Graduated microfiber prefilter from 1 μm to 0.7 μm
  • Higher flow rates (3×) compared to unprotected membranes
  • Uses glass microfiber-based prefilters
For metals testing and other applications where glass-based compounds could interfere with analysis, we offer a related syringe filter (GD/XP), which uses polypropylene prefilters.

Typical data

Whatman GD/X Syringe Filters

Membrane

GD/X 13 mm

GD/X 25 mm

HousingPolypropylene (pigment-free)Polypropylene (pigment-free)
Filtration area1.3 cm24.6 cm2
Maximum pressure100 psi (6.9 bar)75 psi (5.2 bar)
Volume “hold-up” full housing with air purge0.5 ml 50 μl (approx)1.4 ml 250 μl (approx)
Dimensions21.6 × 29.8 mm20.8 × 29.8 mm
Weight3 g (approx)3 g (approx)
Flow directionFlow should enter from the inletFlow should enter from the inlet
Inlet connectionFemale Luer lockFemale Luer lock
Outlet connectionMale LuerMale Luer
Sterilization*Autoclave at 121°C at 15 psi for 20 minAutoclave at 121°C at 15 psi for 20 min
Glass microfiber prefiltration media100% borosilicate GMF 150: 1 μm GF/F 0.7 μm100% borosilicate GMF 150: 1 μm GF/F 0.7 μm
*Applies to nonsterile filters only. Do not autoclave sterile GD/X filters.

Typical data

GD/XP Syringe Filters

 

GD/XP 13 mm

HousingPolypropylene (pigment-free)
Filtration area4.6 cm2
Maximum pressure75 psi (5.2 bar)
Volume “hold-up” full housing1.4 ml with air purge 250 μl (approx)
Dimensions20.8 × 29.8 mm
Weight3 g (approx)
Flow directionFlow should enter from the inlet
Inlet connectionFemale Luer lock
Outlet connectionMale Luer
SterilizationAutoclave at 121°C at 15 psi for 20 min
Prefiltration mediaPP 20 μm: 5 μm
Not recommended for nylon.
Whatman_GDX_Diagram

Whatman GD/X 13mm diameter

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Whatman GD/X 25mm diameter

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Whatman GD/XP 25mm diameter

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Mini-UniPrep™

Autosampler and workflow support: Mini-UniPrep filters

Whatman Mini-UniPrep Syringeless Filters - polypropylene or glass chambers

Mini_UniPrep

The Mini-UniPrep syringeless filters are compatible with most autosamplers.

  • Easy-to-use design supports sample preparation outside of the lab if needed
  • Process samples in one-third the time of traditional syringe filtration
  • Replaces syringe, syringe filter, vial, and cap in one consumable
  • Polypropylene or glass chamber options to prevent interference from chemical leaching
  • Amber vials available for light sensitive samples
  • Multi-compressors available for ease of use
  • 12 × 33 mm vial can be used to filter up to 400 μL
Mini_UniPrep_Parts

Typical data

Mini-UniPrep and Mini-UniPrep G2 filter vials

 

Mini-UniPrep

Mini-UniPrep G2

DimensionsEquivalent in size to 12 mm × 32 mm vialsEquivalent in size to 12 × 32 mm vials
Materials of construction  
Chamber:PolypropyleneBorosilicate glass
Plunger housing:PolypropylenePolypropylene
Plunger inner storage vialN/ABorosilicate glass/td>
Filter medium:As specifiedAs specified
Septum:Silicone with PTFE linerSilicone with PTFE liner
Cap:PolypropylenePolypropylene
Maximum operating temp50°C (122°F)50°C (122°F)
Max. unfiltered sample capacity400 μL500 μL
Max. filtered sample capacity350 μL330 μL
Dead volume50 μL170 μL
Recommended minimum filtering volume100 μL220 μL placed in the chamber to obtain 50 μL in inner storage vial
Nominal force needed to compressApprox. 8.2 kg (18 lbs)Approx. 11.3 kg (25 lbs)
Autosampler needle height adjustment:3 mm from the bottom of Mini-UniPrep5 mm from the bottom of Mini-UniPrep G2
Autosampler compatibilityAny autosampler that accommodates standard 12 × 32 mm profile vialsAny autosampler that accommodates standard 12 × 32 mm profile vials

Mini-UniPrep Compressors

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Mini-UniPrep filter vial polypropylene housing 0.2um pore size

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Mini-UniPrep filter vial polypropylene housing 0.45um pore size

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Mini-UniPrep G2 filter vials with inner glass storage vial 0.2um pore size

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Mini-UniPrep G2 filter vials with inner glass storage vial 0.45um pore size

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