Western Blotting Reagents, Membranes, Supplies

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent  provides highly intense signal output, for detection of medium to very low protein levels.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls

Use for the best results with fluorescence Western blotting detection applications. Cytiva Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Thermo Scientific™ 1-Step™ NBT/BCIP Substrate Solution

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

Merck Millipore Immobilon™-FL PVDF Transfer Membranes

Designed specifically for Western blots and protein sequencing. Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45μm pores and have lower background fluorescence than other membranes. Available in 10cm and 20cm squares and a 3.75m roll.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.

Invitrogen™ XCell II™ Blot Module

Easy transfer of proteins or nucleic acids from mini-gels to membranes

Invitrogen™ Invitrolon™ PVDF/Filter Paper Sandwiches, 0.45 µm, 8.3 x 7.3 cm (for mini gels)

Pre-cut and pre-assembled and ready for protein blotting/transfer

Merck Millipore Immobilon™-P PVDF Transfer Membranes

Optimized for binding proteins transferred from a variety of gel matrices. Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.

Thermo Scientific™ TMB Substrate Solution

A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Hybond™ P 0.45μm PVDF Membrane

Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. Cytiva Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing.

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) HRP substrate with very stable light output for femtogram-level detection with CCD-based imagers.

Merck Millipore Luminata™ Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Invitrogen™ Blotting Roller

Compatible with E-PAGE™, Novex Mini Gels, and NuPAGE™ Gels

Thermo Scientific™ 1-Step™ Chloronaphthol Substrate Solution

1-Step™ Chloronaphthol Substrate Solution

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Hybond™ -N+ Membranes

Use for the highest sensitivity in DNA and RNA blotting. Cytiva Life Sciences™ Amersham™ Hybond™ -N+ Membrane is a positively charged nylon membranes for use with radioactive and nonradioactive chemiluminescence and chemifluorescence detection.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Protran™ NC Nitrocellulose Membranes: Sheets

Use for Western blots, dot-blot assays, and other protein or nucleic acid methods. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Sheets offer excellent sensitivity, resolution, and low background.

Cytiva (Formerly GE Healthcare Life Sciences) Whatman™ Grade GB003 Blotting Paper

Ensures that no contamination will occur during the transfer steps. Whatman™ Grade GB003 Blotting Paper eliminates sheet to sheet variations with precisely cut membrane sizes.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Protran™ Supported NC Nitrocellulose Membranes: Rolls

Use for robust protein blotting. Cytiva Life Sciences™ Amersham™ Protran™ Supported NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Invitrogen™ Blotting Filter Papers, 2.5-mm thick, 8.6 x 13.5 cm

Make blotting of E-PAGE™ Gels easier and more efficient

Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution

Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.

Invitrogen™ WesternBreeze™ Blocker/Diluent (Part A and B)

Optimized, easy-to-use blocker and primary antibody diluent system

Invitrogen™ Blotting Filter Papers, 2.5-mm thick, 7.5 x 8.4 cm

Make blotting of E-PAGE™ Gels easier and more efficient

Thermo Scientific™ 1-Step™ NBT/BCIP plus Suppressor Substrate Solution

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

Thermo Scientific™ HisProbe™-HRP Conjugate

Directly detect histidine-tagged fusion proteins and other histidine-rich proteins with this nickel-activated horseradish peroxidase (HRP) derivative.

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