Protein Isolation and Purification

Triton™ X-100 (Electrophoresis), Fisher BioReagents™

CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 IUPAC Name: 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium Lauryl Sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

Cytiva (Formerly GE Healthcare Life Sciences) PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight  >5000 Mr in sample volumes up to 0.5mL.  Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

SDS Solution, 10% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

Cytiva (Formerly GE Healthcare Life Sciences) LabMate™ PD-10 Buffer Reservoirs

Use for high-recovery desalting, buffer exchange and removal of low-molecular weight compounds packed with Sephadex™ G-25 Medium. Cytiva Life Sciences™ LabMate™ PD-10 Buffer Reservoirs provide fast, affordable performance.

Cytiva (Formerly GE Healthcare Life Sciences) Thrombin Protease

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

Cytiva (Formerly GE Healthcare Life Sciences) StrepTactin™ Sepharose™ High Performance Medium

Mild purification conditions preserve antigenicity and function. Cytiva StrepTactin Sepharose High Performance media is used for robust, high resolution purification of Strep II-tagged proteins.

Cytiva (Formerly GE Healthcare Life Sciences) Chelating Sepharose™ Fast Flow Medium

Use for purifying native and recombinant proteins and peptides that have an affinity for metal ions. Chelating Sepharose™ Fast Flow Medium provides robust performance and regulatory support.

Thermo Scientific™ Low Protein Binding Collection Tubes (1.5 mL)

Minimize protein sample loss during sample manipulation, especially when there are many processing steps, by using Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (1.5 mL).

Cytiva (Formerly GE Healthcare Life Sciences) HiTrap™ Capto™ Core 700 Columns

HiTrap Capto Core 700 is prepacked with Capto Core 700, a multimodal BioProcess medium for intermediate purification and polishing of viruses and other large biomolecules.

Cytiva (Formerly GE Healthcare Life Sciences) HiScreen Capto™ Core 700 Columns

Capto Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules.

Thermo Scientific™ Low Protein Binding Collection Tubes (2.0 mL)

Minimize protein sample loss during sample manipulation, especially when there are many processing steps, by using Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (2.0 mL).

Cytiva (Formerly GE Healthcare Life Sciences) PreScission™ Protease

Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.

Macherey-Nagel™ NucleoVac Vacuum Regulator

Ensures a reliable vacuum source when manually processing DNA columns under vacuum. Macherey-Nagel™ NucleoVac Vacuum Regulator is designed for use with the NucleoVac Vacuum Manifold for fast processing of large sample volumes via parallel processing of columns.

SDS Solution, 20% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium lauryl sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

Cytiva (Formerly GE Healthcare Life Sciences) Tricorn Columns

Tricorn columns are compatible with high flow rates and are designed for high-performance media such as Sepharose, Superdex, and SOURCE.

Macherey-Nagel™ NucleoSpin™ TriPrep

NucleoSpin™ miRNA is suitable for the parallel isolation of small RNA, large RNA and protein

Izon qEVoriginal SEC Columns

qEV provides the most rapid and effective EV/exsosome isolation system available. qEV is based on Size Exclusion Chromatography (SEC), and takes around 15 minutes to get a pure sample of intact exosomes. The resulting samples are consistent, standardisable and repeatable.

IBA Lifesciences™ Strep-Tactin™ Sepharose™ Suspension

Ideal for gravity flow purification of recombinant Strep-tag™ proteins. IBA Lifesciences™ Strep-Tactin™ Sepharose™ Suspension is conveniently formulated as a 50% Sepharose™ suspension.

Cytiva (Formerly GE Healthcare Life Sciences) XK Chromatography Columns (Empty)

Ensure trouble-free operation for low to medium pressure. Cytiva XK Chromatography Columns (Empty) connect directly to ÄKTA design instruments or other liquid chromatography systems. The user-friendly ensures reproducible, accurate packing.

Izon qEVsingle Single-use Columns

qEV provides the most rapid and effective EV/exsosome isolation system available. qEV is based on Size Exclusion Chromatography (SEC), and takes around 15 minutes to get a pure sample of intact exosomes. The resulting samples are consistent, standardisable and repeatable.

Macherey-Nagel™ NucleoSpin™ RNA/Protein Column

Isolate RNA and protein from undivided samples. Macherey Nagel™ NucleoSpin™ RNA/Protein Column Set solves the problem of extracting both protein and RNA from the same sample in parallel

Cytiva (Formerly GE Healthcare Life Sciences) PD MultiTrap™ Plates

Convenient and fast small-scale cleanup of proteins and other large biomolecules (>5000 Mr) with high reproducibility from well to well and from plate to plate

Cytiva (Formerly GE Healthcare Life Sciences) Tricorn™ Filter Kit

Used for large volume/repeated loading of clarified sample to reduce the risk of clogging in combination with capture media.  Cytiva Tricorn™ Filter Kit includes top and bottom adapters, bottom filters, and EPDM O-rings, five of each  

Thermo Scientific™ SMART Digest™ Trypsin Kits

Obtain fast, simple, and highly reproducible trypsin protein digestion for peptide characterization and quantitation in biopharmaceutical protein research.

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