Protein Extraction and Purification

Thermo Scientific™ Pierce™ RIPA Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent. RIPA BUFFER, 250 MLlysis and extraction buffer 250mL Store at 4C,

Thermo Scientific™ Pierce™ LAL Chromogenic Endotoxin Quantitation Kit

Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay. PIERCE LAL CHROMOGENIC ENDOTOXIN QUANTITATION

Triton™ X-100 (Electrophoresis), Fisher BioReagents™

CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 IUPAC Name: 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO 500ML Triton X-100 for Electrophoresis

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium Lauryl Sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+] 5KG Sodium Dodecyl Sulfate (SDS), Electrophoresis(White Powder), (as Fatty Alcohol Sulfate):

Invitrogen™ Magnetic Stand-96

Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories MAGNETIC STAND-96 1 STANDFor the paramagnetic bead precipitation from

Thermo Scientific™ Pierce™ DNase I

Improve protein extraction efficiency with this glycosylated polypeptide that cleaves single- and double-stranded DNA in cell lysates. DNASE

Thermo Scientific™ Pierce Protease Inhibitor Tablets

Thermo Scientific™ Pierce Protease Inhibitor Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments LG PROTEASE INH SAMPLE 1 VIAL

Thermo Scientific™ Triton X-100 Detergent Surfact-Ams™ Solution

Thermo Scientific™ Surfact-Amps X-100 Detergent Solution is highly-purified Triton X-100 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

1000 ML TRITON X-100 SURFACT-AMPS DETERGENT SOLUTIon 1000mL Store at Room temperature

GE Healthcare PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight  >5000 Mr in sample volumes up to 0.5mL.  GE Healthcare Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked. X50 PD MINITRAP G-25

Merck™ BugBuster™ 10X Protein Extraction Reagent

Reagent for protein sample preparation 50ML BugBuster Protein Extraction Reagent

GE Healthcare HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. GE Healthcare HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). HISTRAP HP 5ML

Thermo Scientific™ Pierce™ Microcentrifuge Spin Cups and Spin Columns

Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500µL). X50 SPIN CUPS CELLUL ACETATE FILT

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments PROTEASE INH EDTA-FREE SAMPLE 1 VIAL

SDS Solution, 10% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+] 1LT Sodium Dodecyl Sulfate (SDS) 10% Solution (Electrophoresis, DNase RNase & protease free)

Thermo Scientific™ Halt™ Protease Inhibitor Cocktails

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors. 5 ML HALT(TM) PROTEASE INHIBITOR COCKTAIL, EDTA-FRee 100x 5mL 4C,Ice Pack Store in Fridge at 4C

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates. HISPUR NI-NTA SETTLED RESIN

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktails

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors. HALT PROTEASE&PHOSPHAT INHIB 10ML

Invitrogen™ MagnaRack™ Magnetic Separation Rack

Holds up to 24 microcentrifuge tubes MAGNARACK 24each

ExpiSf™ CD Medium

MEDIUM INSECT GIBCO 10 L

Thermo Scientific™ P-PER™ Plant Protein Extraction Kit

Effectively extract protein from all kinds of dry and fresh plant tissue without liquid nitrogen using this plant cell-optimized kit. 1 SET P-PER PLANT PROTEIN EXTRACTIO

GE Healthcare Gel Filtration Calibration Kits

Calibrate gel filtration columns simply, reliably. GE Healthcare Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards. GEL FILTRATION CAL KIT LMW

Invitrogen™ 24 Deep-Well RB Block

Designed for experimental protocols that require volumes exceeding the capacity of conventional shallow well plates 24 DEEP WELL RND BTM BLOCKThe 24 Deep Well RB Block is designed for

Thermo Scientific™ Pierce™ Centrifuge Columns

Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes. X10 H&EE COLUMN EXTENDER

Thermo Scientific™ Pierce™ Disposable Plastic Columns

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques. X50 Funnel Pierce disposablepolypropylene (pack

GE Healthcare HisTrap™ FF Crude Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. GE Healthcare Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). X5 HISTRAP FF CRUDE 5x 5ML Prepacked Columns

Merck™ Novagen™ Benzonase™ Nuclease, >99%

Degrades all forms of single strand, double stranded, linear, circular DNA RNA. BENZONASE, PURITY, MAIOR 99%, SIZE = 10 KU

IBA GmbH™ 10x Buffer BXT; Strep-Tactin™XT Elution B.

IBA GmbH™ 10x Buffer BXT; Strep-Tactin™XT Elution B. is a ready-to-use buffer for elution of Strep-tagged proteins from Strep-Tactin™ XT. 25ML 10x Buffer BXT; Strep-TactinXT Elution Buffer

GE Healthcare Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. GE Healthcare Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification. Prepacked column, Superdex 200 Increase 10/300 GL

GE Healthcare LabMate™ PD-10 Buffer Reservoirs

Use for high-recovery desalting, buffer exchange and removal of low-molecular weight compounds packed with Sephadex™ G-25 Medium. GE Healthcare Life Sciences™ LabMate™ PD-10 Buffer Reservoirs provide fast, affordable performance. X10 Buffer reservoir GE Healthcare for Labmate

GE Healthcare HisTrap™ FF Columns: 5 x 5mL

Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media. X5 HISTrap Fast flow 5mL

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