Protein Extraction and Purification

Invitrogen™ Magnetic Stand-96

Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories MAGNETIC STAND-96 1 STANDFor the paramagnetic bead precipitation from

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium Lauryl Sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+] 5KG Sodium Dodecyl Sulfate (SDS), Electrophoresis(White Powder), (as Fatty Alcohol Sulfate):

Thermo Scientific™ Pierce™ RIPA Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent. RIPA BUFFER, 250 MLlysis and extraction buffer 250mL Store at 4C,

GE Healthcare Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. GE Healthcare Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification. Prepacked column, Superdex 200 Increase 10/300 GL

GE Healthcare HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. GE Healthcare HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). HISTRAP HP 5ML

Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit

A highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acids or antibody sample using the Amebocyte Lysate Assay. 30RXN Chromogenic Endotoxin Quant Kit

Thermo Scientific™ Pierce™ LAL Chromogenic Endotoxin Quantitation Kit

Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay. PIERCE LAL CHROMOGENIC ENDOTOXIN QUANTITATION

Triton™ X-100 (Electrophoresis), Fisher BioReagents™

CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 IUPAC Name: 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO 500ML Triton X-100 for Electrophoresis

Thermo Scientific™ Pierce Protease Inhibitor Tablets

Thermo Scientific™ Pierce Protease Inhibitor Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments X20 Pierce Protease Inhibitor Tablets, EDTA-Free

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments X30 Pierce Protease Inhibitor Mini Tablets

GE Healthcare HisTrap™ FF Crude Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. GE Healthcare Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). X100 HisTrap(TM) FF crude, ready to use column,

Merck™ BugBuster™ 10X Protein Extraction Reagent

Reagent for protein sample preparation X10 BUGBUSTER 10X, SIZE =

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates. X5 HISPUR NI-NTA PURIFICATION KIT

Invitrogen™ MagnaRack™ Magnetic Separation Rack

Holds up to 24 microcentrifuge tubes MAGNARACK 24each

SDS Solution, 20% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium lauryl sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+] 200ML Sodium Dodecyl Sulfate (SDS), 20% Solution (Electrophoresis), (as Fatty Alcohol Sulfate):

Thermo Scientific™ Pierce™ Centrifuge Columns

Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes. X200 CENTRIFUGE COLUMNS, 0,8 ML

Thermo Scientific™ Pierce™ Disposable Plastic Columns

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques. COLUMN PIERCE PLUS 2 EACH OF PN29920, PN29922,PN29924 and 1 of PN29923 (trial pack) disposable

Invitrogen™ eBioscience™ RBC Lysis Buffer, 1x

Formulated for optimal lysis of erythrocytes in single-cell suspensions of mouse hematopoietic tissues such as spleen and human peripheral blood. eBioscience 1X RBC LysisBuffer 200 ml

GE Healthcare Streptavidin Sepharose™ High Performance Medium

Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium. STREPTAVIDIN SEPHAROSE HP,5 ML

Merck™ Novagen™ Benzonase™ Nuclease, >99%

Degrades all forms of single strand, double stranded, linear, circular DNA RNA. BENZONASE, PURITY, MAIOR 99%, SIZE = 10 KU

GE Healthcare Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophobic

Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds. 100mL SpeedBead magnetic carboxylate modified particles,DSMG-CM, 1 um, 5% solids

GE Healthcare Sephadex™ G-25 Media

Use of Sephadex™ is  well established as a gel filtration medium for desalting and buffer exchange in industrial applications. GE Healthcare Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step. SEPHADEX G-25 FINE 500 GRS

SDS Solution, 10% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+] 1LT Sodium Dodecyl Sulfate (SDS) 10% Solution (Electrophoresis, DNase RNase & protease free)

Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells

Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours. SUBCELLULAR PROTEIN FRACTIONATION

Invitrogen™ Single Place Magnetic Stand

Accommodates one 1.5mL microfuge tube SINGLE PLACE MAGNETIC STANDone 1.5 mL microfuge tube

Thermo Scientific™ Halt™ Protease Inhibitor Cocktails

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors. 10 ML HALT(TM) PROTEASE INHIBITOR COCKTAIL, EDTA-Free 100x 10mL

GE Healthcare Gel Filtration Calibration Kits

Calibrate gel filtration columns simply, reliably. GE Healthcare Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards. GEL FILTRATION CAL KIT HMW

Thermo Scientific™ Pierce Protease and Phosphatase Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease and Phosphatase Inhibitor Mini Tablets broad-spectrum protease and pghospahatase inhibitor formulation provides complete protection from dephosphorylation and proteolytic degradation. X20 Pierce Protease and Phoshpatase Inhibitor Mini Tablets

IBA Solutions for Life Science™ MagStrep “type 3” XT Beads 5% Suspension

Have high binding capacity combined with very low non-specific protein binding 2ML MagStrep ''type3'' XT beads , 5% suspension

GE Healthcare HiTrap™ Heparin HP Columns

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. GE Healthcare Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification. X5 HITrap Heparin High performance 5mL

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