Enzymes and Inhibitors

Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA). 1 LT 50X TAE BUFFER (TRIS-ACETATE-EDTA), 1X CONCENtration includes 40 mM Tris, 20 mM acetic acid, 1

Thermo Scientific™ Bovine Serum Albumin (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin. 250 UL BOVINE SERUM ALBUMIN (BSA), 20MG/ML, 5MG(0.25mL), supplied in 10 mM Tris-HCl (pH7.4 at

Thermo Scientific™ FastDigest™ BamHI

Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. X5 FASTDIGEST BAMHI 500UL

Thermo Scientific™ FastDigest™ EcoRI

The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer. X5 FASTDIGEST ECORI 500UL

Thermo Scientific™ FastDigest KpnI

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. FASTDIGEST KPNI 300UL

Thermo Scientific™ FastDigest NheI

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. 100 UL RESTRICTION ENZYME, FASTDIGEST, NHEI, CUTSG¬CTAGC, 1µL cleaves 1µg DNA in 5 minutes at 37°C

Thermo Scientific™ FastDigest MscI (MlsI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest MlsI, 50µL

Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA)

Perform electrophoresis of nucleic acids in agarose and polyacrylamide gels using Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA). TBE BUFFER 1L 10X

Thermo Scientific™ FastDigest™ DpnI

Cut at Gm6A^TC sites with Thermo Scientific™ FastDigest™ DpnI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: MalI). FASTDIGEST DPNI 50UL

Thermo Scientific™ NcoI

The NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: Bsp19I). 2500 UNIT RESTRICTION ENZYME, CONVENTIONAL, NCOI,10u/uL, cuts C¬CATGG at 37°C (suppliedwith 1mL of

Thermo Scientific™ FastDigest BsmBI (Esp3I)

Cut at CGTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ BsmBI (Esp3I), which peforms best at 37°C in 5-15 minutes using universal FastDigest Buffer. Restriction enzyme, FastDigest Esp3I, 20µL

Thermo Scientific™ BpiI (BbsI)

Cut GAAGAC(2/6)^ sites with BpiI (BbsI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: BbsI, BpuAI, BstV2I). 200 UNIT RESTRICTION ENZYME, CONVENTIONAL, BPII(BbsI), 10u/ul, cuts GAAGAC(2/6)¬ at 37°C (supplie

Thermo Scientific™ FastDigest™ SpeI (BcuI)

FastDigest SpeI (BcuI) restriction enzyme recognizes A^CTAGT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AhlI). Restriction enzyme, FastDigest BcuI, 50µL

Thermo Scientific™ 10x Buffer Tango

Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer Tango, premixed with BSA for enhanced stability. X5 10X Buffer Tango Unit Size 5x1.0 mL

Thermo Scientific™ FastDigest MluI

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. FASTDIGEST MLUI 100UL

Thermo Scientific™ FastDigest PacI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. 25 UL RESTRICTION ENZYMES, ,FastDigest PacI, 25µL, cuts TTAAT¬TAA, 1µL cleaves

Thermo Scientific™ PstI

The PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI). PSTI 10U/UL 10000U

Thermo Scientific™ FastDigest™ XhoI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. X3 FASTDIGEST XHOI 400UL

Thermo Scientific™ FastDigest ApaI

Cut at GGGCC^C sites with Thermo Scientific™ FastDigest™ ApaI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PspOMI). FASTDIGEST APAI 300UL

Thermo Scientific™ FastDigest PvuI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. FASTDIGEST PVUI 20UL

Thermo Scientific™ Eco31I (BsaI)

The Eco31I (BsaI) restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in G buffer (Isoschizomers: BsaI, Bso31I, BspTNI). ECO31I (BSAI) 10U/UL 5000U

Thermo Scientific™ EcoRI

The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer. ECORI 50U/UL 25000U

Thermo Scientific™ Buffer R

Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer R, premixed with BSA for enhanced stability. 10X BUFFER R 5X1 ML

Thermo Scientific™ ATP

Optimize many molecular biology applications using this ATP, a highly stable nucleotide with 99% purity. ATP 100MM 0.25ML

Thermo Scientific™ FastDigest™ PstI

The FastDigest PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BspMAI). X5 FASTDIGEST PSTI 500UL

Thermo Scientific™ 10X T4 DNA Ligase Buffer

Safely ligate samples with this buffer, for use with T4 DNA Ligase and tested for the absence of endo- and exodeoxyribonucleases and ribonucleases. X10 T4 DNA LIGASE 1.5ML

Thermo Scientific™ FastDigest™ SmaI

The FastDigest SmaI restriction enzyme recognizes CCC^GGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: TspMI, XmaCI; neoschizomer: XmaI) FASTDIGEST SMAI 200UL

Thermo Scientific™ BamHI

The BamHI restriction enzyme recognizes G^GATCC sites and cuts best at 37°C in its own unique buffer. BAMHI 10U/UL 10000U

Thermo Scientific™ FastDigest™ HindIII

The FastDigest HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer. X5 FASTDIGEST HINDIII 500UL

Thermo Scientific™ Esp3I (BsmBI)

The Esp3I (BsmBI) restriction enzyme recognizes CGTCTC(1/5)^ sites and cuts best at 37°C in Tango (+DTT) buffer (Isoschizomers: BsmBI). ESP3I (BSMBI) 10U/UL 1000U

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