Thermo Scientific™ Exonuclease I (20 U/μL)

Product Code.	Quantity	unitSize
10548930	4,000 units	4000 units
10354530	20,000 units	20000 units

Product Code. 10548930

Brand: Thermo Scientific™ EN0581

Description

Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.

Exonuclease I (ExoI) degrades single-stranded DNA in a 3'≥5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.

Highlights

• Active in PCR buffers

Applications

• Primer removal from PCR mixtures:
  • prior to PCR product sequencing
  li>for one-tube "megaprimer" PCR mutagenesis
• Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
• Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus

Note

The enzyme is not suitable for removing 3'-overhangs of dsDNA.

Specifications

• Concentration: 20 U/μL
• Enzyme: Exonuclease
• Quantity: 4,000 units
• Product Type: Exonuclease I

Safety and Handling

Product Identifier

• Exonuclease I

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.

Supplemental information

• MIXTURE LIST-Contains : Potassium hydroxide

For Research Use Only. Not for use in diagnostic procedures.