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  5. Thermo Scientific™ Exonuclease I (20 U/μL)

Thermo Scientific™ Exonuclease I (20 U/μL)

Product Code. 10548930 Shop All Thermo Scientific Products
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Quantity:
20,000 units
4,000 units
Unit Size:
20000 units
4000 units
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Product Code. 10548930

Brand: Thermo Scientific™ EN0581

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67.10 GBP valid until 2025-12-19
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Description

Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.

Exonuclease I (ExoI) degrades single-stranded DNA in a 3'≥5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.

Highlights

  • Active in PCR buffers

Applications

  • Primer removal from PCR mixtures:
    • prior to PCR product sequencing
      • li>for one-tube "megaprimer" PCR mutagenesis
  • Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
  • Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus

Note

The enzyme is not suitable for removing 3'-overhangs of dsDNA.

TRUSTED_SUSTAINABILITY

Specifications

Concentration 20 U/μL
Enzyme Exonuclease
Quantity 4,000 units
Product Type Exonuclease I

Safety and Handling

compliance-icons
Product Identifier
  • Exonuclease I
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
 Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
 Precautionary Statement
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P362+P364-Take off contaminated clothing and wash it before reuse.
 Supplemental information
  • MIXTURE LIST-Contains : Potassium hydroxide

For Research Use Only. Not for use in diagnostic procedures.

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