Thermo Scientific™ DNase I, RNase-free

An endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: EN0523

DNASE I RNASE-FRE 50U/UL 1000U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 10272490

Quantity Price
1 £ 45.76 / 1000 units
EU Stock 20
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Description and Specification

Specification

Concentration 50U/µL
Inhibitors metal chelators, transition metals (e.g., Zn) in millimolar concentrations, SDS (even at concentrations less than 0.1%), reducing agents (DTT and beta-mercaptoethanol), ionic strength above 50-100 mM
Source E.coli cells with a cloned gene encoding bovine DNase
Molecular Weight 29 kDa monomer
Quantity 50U/µL
Units 1,000U

Highlights

  • Recombinant enzyme
  • Purified from non-animal host with a lower level of intrinsic RNases

Applications

  • Preparation of DNA-free RNA
  • Removal of template DNA followingin vitrotranscription
  • Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
  • DNA labeling by nick-translation in conjunction with DNA Polymerase I
  • Studies of DNA-protein interactions by DNase I, RNase-free footprinting
  • Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used

Note

DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.