Western Blotting Reagents, Membranes, Supplies
GE Healthcare Amersham™ Hyperfilm™ ECL
Use with all blue and green light chemiluminescent systems. GE Healthcare Amersham™ Hyperfilm™ ECL is constructed from a clear base coated on both sides with a photographic emulsion that is protected by an anti-scratch layer.
Invitrogen™ iBlot™ 2 Transfer Stacks, nitrocellulose, mini
Used to transfer proteins with the iBlot 2 Gel Transfer Device
GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent
Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity. GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.
GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls
Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate
Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.
Thermo Scientific™ Pierce™ ECL Western Blotting Substrate
Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.
Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Invitrogen™ XCell SureLock™ Mini-Cell and XCell II™ Blot Module
Quickly and easily run and blot Novex minigels
Thermo Scientific™ Western Blotting Filter Papers, 0.83-mm thick, 8 x 13.5 cm
Pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels to PVDF, nitrocellulose, or other membranes.
Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis
GE Healthcare Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls
Use for the best results with fluorescence Western blotting detection applications. GE Healthcare Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
Invitrogen™ iBlot™ 2 Transfer Stacks, PVDF, mini
Consumable stacks with integrated PVDF transfer membranes for dry blotting of proteins
Thermo Scientific™ Restore™ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
Thermo Scientific™ CL-XPosure™ Film, 8 x 10 in. (20 x 25 cm)
Capture results of ECL-based Western blots and other chemiluminescent protein or nucleic acid assays using this economically priced, clear-blue X-ray film.
Thermo Scientific™ Pierce™ 1-Step Transfer Buffer
Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.
Thermo Scientific™ 1-Step™ NBT/BCIP plus Suppressor Substrate Solution
Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.
Invitrogen™ Nitrocellulose/Filter Paper Sandwiches, 0.45 µm, 8.3 x 7.3 cm
Ideal for blotting proteins and nucleic acids
Merck Millipore Immobilon™-FL PVDF Transfer Membranes
Designed specifically for Western blots and protein sequencing. Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45μm pores and have lower background fluorescence than other membranes. Available in 10cm and 20cm squares and a 3.75m roll.
Thermo Scientific™ PVDF Transfer Membranes
Enable high protein and nucleic acid binding capacity with sheets and rolls of high-quality polyvinylidene difluoride (PVDF) membrane.
GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents
Use for all routine Western blotting. GE Healthcare Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.
Invitrogen™ XCell II™ Blot Module
Easy transfer of proteins or nucleic acids from mini-gels to membranes
Thermo Scientific™ Blocker™ Casein
Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods.
Invitrogen™ Mini Gel Tank and Blot Module Set
Provides all of the necessary devices for protein separation and Western blotting using Novex™ gels, NuPAGE™ gels, and Bolt™ Bis-Tris Plus gels
Invitrogen™ Invitrolon™ PVDF/Filter Paper Sandwiches, 0.45 µm, 8.3 x 7.3 cm (for mini gels)
Pre-cut and pre-assembled and ready for protein blotting/transfer
Merck Millipore Immobilon™ Western Chemiluminescent HRP Substrate (ECL)
Provides high sensitivity over a broad detection range at an economical price
Merck Millipore Immobilon™-P PVDF Transfer Membranes
Optimized for binding proteins transferred from a variety of gel matrices. Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.
Thermo Scientific™ TMB Substrate Solution
A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)
GE Healthcare Amersham™ Hybond™ P 0.45μm PVDF Membrane
Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. GE Healthcare Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing.
Thermo Scientific™ Nitrocellulose Membrane, 0.45 µm, 7.9 cm x 10.5 cm
Sheets and rolls of nitrocellulose, available in two pore sizes, with excellent binding properties for Western blot and other protein or nucleic acid methods.
Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate
Luminol-based enhanced chemiluminescence (ECL) HRP substrate with very stable light output for femtogram-level detection with CCD-based imagers.