Filtered Search Results
Applied Biosystems™ TaqMan™ Gene Expression Assay (FAM)
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.
| Shipping Condition | Room Temperature |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 60 X |
| Product Type | Gene Expression Assay |
| Reaction Speed | Fast |
| Green Features | Sustainable packaging |
| PCR Method | qPCR |
| Product Line | TaqMan™ |
| Detection Method | Primer-probe |
Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
| GC-Rich PCR Performance | High |
|---|---|
| Format | Kit |
| Reverse Transcriptase | SuperScript IV |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 10 min. |
| Reagent Type | Reverse Transcription |
| Quantity | Each |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Applied Biosystems™ TaqMan™ Universal PCR Master Mix
TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.
| Polymerase | AmpliTaq Gold DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Product Type | Universal PCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
| PCR Method | qPCR |
| Product Line | TaqMan™ |
| Detection Method | Primer-probe |
Axygen™ PCR Tubes with 0.5 mL Flat Cap
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Thin and consistent wall thickness for precise thermal transfer
| Format | Tube |
|---|---|
| Shape | Conical |
| For Use With (Equipment) | Standard 96-well blocks of real-time PCR instruments and thermal transfer applications |
| Volume (Metric) | 0.5 mL |
| Material | Polypropylene |
| Certifications/Compliance | RNase, DNase, pyrogen-free |
| Closure Type | Flat Cap |
| Sterility | Non-sterile |
| Closure Material | Polypropylene |
Invitrogen™ M-MLV Reverse Transcriptase (200 U/μL)
M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid.
| Shipping Condition | Wet Ice |
|---|---|
| Format | Stand-alone Enzyme |
| Reverse Transcriptase | M-MLV |
| Size (Final Product) | Up to 7 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 50 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Yes |
| Optimal Reaction Temperature | 37°C |
| Technique | Reverse Transcription |
| Concentration | 200 U/μL |
| Starting Material | RNA |
Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | Maxima H Minus |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 20 kb |
| Reaction Format | Separate components |
| Reaction Speed | 15 to 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C to 55°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Thermo Scientific™ Exo-Resistant Random Primer
Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions.
Invitrogen™ Taq DNA Polymerase, recombinant
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Standard PCR |
| PCR Method | qPCR,Standard PCR |
| Exonuclease Activity | 5' - 3' |
| Polymerase | Taq DNA Polymerase |
| Format | Stand-alone enzyme |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | No |
| Starting Material | DNA |
Thermo Scientific™ CloneJET PCR Cloning Kit
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
| Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
|---|---|
| Format | Kit |
| Sample Type | PCR Products |
| Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
| For Use With (Application) | PCR Cloning |
| Vector | pJET1.2/blunt |
| Product Line | CloneJET |
Eppendorf™ PCR Clean Colorless Safe-Lock Centrifuge Tubes
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Protect your samples with these centrifuge tubes. Eppendorf™ PCR Clean Colorless Safe-Lock Centrifuge Tubes feature a hinged Eppendorf Safe–Lock lid for outstanding protection against unintentional opening during incubation and storage.
| Product Type | Centrifuge Tube |
|---|---|
| Color | Natural |
| Material | Polypropylene |
| Autoclavable | Autoclavable |
| Closure Included | Yes |
Eppendorf™ PCR Tubes - Singles and Strips
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Eppendorf PCR Tubes ensure uniform heat distribution to the sample for optimal PCR performance. The user-friendly design allows for easy opening and provides a secure seal to prevent evaporation.
| Sterility | Non-sterile |
|---|
Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0
Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.
| Purity or Quality Grade | UP (Ultra Pure) |
|---|---|
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Genotyping,Pathogen Detection,Virus Detection |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Format | Bottle |
| Product Type | qPCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode |
| Sample Type | Environmental Sample, Food |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Product Line | TaqMan™ |
Thermo Scientific™ PCR Master Mix (2X)
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.
| Polymerase | Taq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Reaction Format | SuperMix or Master Mix |
| Product Type | PCR Master Mix |
| Reaction Speed | Standard |
| Hot Start | No |
| Overhang | 3'-A |
| Concentration | 2X |
| Starting Material | DNA |
| For Use With (Application) | Standard PCR |
Invitrogen™ Oligo(dT)20 Primer
Used for first-strand cDNA synthesis with reverse transcriptase at temperatures of ≥50°C, Oligo(dT)20 Primer is a string of 20 deoxythymidylic acid residues that hybridizes to the poly(A) tail of mRNA.
Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion High-Fidelity DNA Polymerase |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | No |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Standard PCR,High-fidelity PCR |