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Gibco™ PBS, pH 7.4

Gibco PBS, pH 7.4, is a balanced salt solution ideal for cell culture applications. It lacks calcium, magnesium, and phenol red, preventing cell adhesion and assay interference. Trusted for quality, Gibco PBS ensures reliable results in cell washing, transport, and reagent preparation.

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Specifications

Life Science Buffers

Physical Form	Liquid
pH	7.3 to 7.5
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Chemical Name or Material	Phosphate Buffered Saline (PBS)
Recommended Storage	15°C to 30°C
Concentration	1 X
For Use With (Application)	Mammalian Cell Culture
Osmolality	280 - 315 mOsm/kg
Sterility	Sterile-filtered

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Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

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Specifications

Life Science Buffers

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
SMILES	C(C(CO)(CO)N)O
Synonym	Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

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PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™

Commonly used buffer

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Specifications

Life Science Buffers

Physical Form	Liquid
Recommended Storage	Room temperature

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TBS, Tris Buffered Saline, 10X Solution, pH 7.4, Molecular Biology, Fisher BioReagents™

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Specifications

Life Science Buffers

Boiling Point	100°C
Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris Buffered Saline
Grade	Molecular Biology
DNase	DNase free
ChemAlert Storage Symbol	Gray
Name Note	10X Solution
Concentration or Composition (by Analyte or Components)	10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl.
CAS	7732-18-5
Protease	Protease free
pH	7.5
Synonym	TBS
Purity Grade Notes	DNase-, RNase- and Protease-Free
Recommended Storage	RT

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TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™

Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT

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Specifications

Life Science Buffers

Name Note	50X Solution
Concentration or Composition (by Analyte or Components)	Tris (24%), Acetic Acid (5.0%), and EDTA (<2%).
CAS	6850-28-8
Protease	Protease free
Physical Form	Liquid
Grade	Electrophoresis
Synonym	TAE
Purity Grade Notes	DNase-, RNase- and Protease-Free
DNase	DNase free
Recommended Storage	RT
Filtered Through	Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol	Gray

TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents

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Specifications

Life Science Buffers

Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris-EDTA
Grade	Molecular Biology
DNase	DNase free
Filtered Through	Filtered through a 5-micron filter.
ChemAlert Storage Symbol	Gray
Name Note	1X Solution, pH 8.0
CAS	7732-18-5
Protease	Protease free
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0
Health Hazard 2	CAUTION!
pH	7.4 to 8.1
Synonym	TE
Recommended Storage	RT

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Invitrogen™ 4X Bolt™ LDS Sample Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats

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Specifications

Gel Electrophoresis Buffers and Diluents

Buffer	Sample Loading Buffers
Content And Storage	Sample Buffer (4X) containing lithium dodecyl sulfate (LDS) at pH 8.5 with SERVA Blue G250 and phenol red. Store at 4°C to 25°C.
Gel Type	Bis-Tris Plus
Product Type	LDS Sample Buffer
For Use With (Equipment)	Bolt Mini Gel Tank
Concentration	4X
Gel Compatibility	Bolt™ Bis-Tris Plus Gels
Product Line	Bolt™

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Invitrogen™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)

Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels

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Gel Electrophoresis Buffers and Diluents

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Tris(Hydroxymethyl) Methylamine, 'Tris Buffer', 99+%, Extra Pure, SLR, Fisher Chemical™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: 4679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

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Specifications

Life Science Buffers

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	4679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™

Commonly used buffering agent | CAS: 7365-45-9 | C8H18N2O4S | 238.30 g/mol

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Specifications

Life Science Buffers

Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
Color	White
Chemical Name or Material	HEPES
Grade	Molecular Biology
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
Identification	Pass Test
DNase	DNase free
Merck Index	15, 4689
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
ChemAlert Storage Symbol	Gray
Assay Percent Range	≥99 %
PubChem CID	23831
Absorbance	0.01 max. (0.1M solution) at 280nm
Percent Purity	≥99%
CAS	7365-45-9
Protease	Protease free
Health Hazard 3	Emergency Overview Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. . NFPA Health:2 Flammability:1 Instability:1
MDL Number	MFCD00006158
Health Hazard 2	WARNING!
Solubility Information	Soluble in water
pH	5.0 to 6.5
Purity Grade Notes	DNase-, RNase- and Protease-Free
Recommended Storage	RT
Molecular Formula	C8H18N2O4S

PBS, Phosphate Buffered Saline, 1X Solution, pH 7.4, Fisher BioReagents™

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Specifications

Life Science Buffers

Packaging	Poly Cube
Physical Form	Liquid
pH	7.3 to 7.5
Recommended Storage	Room temperature
Melting Point	0°C

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Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™

Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.

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Life Science Buffers

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Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels

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Specifications

Life Science Buffers

Chemical Name or Material	Running Buffer
Concentration	10X
Product Line	Novex

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HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents™

Commonly used buffering agent

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Specifications

Life Science Buffers

Color	Undesignated
Physical Form	Liquid
Chemical Name or Material	HEPES Buffer
Grade	Molecular Biology
Identification	Pass Test
DNase	DNase free
Merck Index	15, 4689
Concentration	0.95 to 1.05 M
ChemAlert Storage Symbol	Gray
Absorbance	≤0.01(0.1M solution) at 260nm,0.004 max. (0.1M solution) at 280nm
Name Note	1M Solution, pH 7.3
CAS	7732-18-5
Protease	Protease free
pH	7.2 to 7.5
Synonym	N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid
Purity Grade Notes	DNase-, RNase- and Protease-Free
Recommended Storage	RT
Molecular Formula	C₈H₁₈N₂O₄S
Formula Weight	238.197

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Tris Hydrochloride, 1M Solution (pH 7.0/Mol. Biol.), Fisher BioReagents™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris-HCl PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing and Availability
Specifications

Life Science Buffers

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
SMILES	C(C(CO)(CO)N)O
Synonym	Tris-HCl
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

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Gibco™ PBS, pH 7.4 Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ PBS, pH 7.4