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DMEM-F-12 Cell Culture Media

DMEM/F-12 cell culture media is a serum-free formulation that combines Dulbecco's modified Eagle medium (DMEM) and Ham's F-12 nutrient mixture in a 1:1 ratio. It is designed to provide a suitable environment for the growth and maintenance of various cell types in laboratory settings. This medium is enriched with essential nutrients, including insulin, transferrin, and growth factors, supporting the optimal proliferation and survival of cells1. DMEM/F-12 cell culture media has been widely used for culturing primary mammalian cells as well as various normal and transformed cell lines. Its versatility and ability to support a broad range of cell types make it a valuable tool in cellular research and biotechnological applications.
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1 – 15 of 28 results
1
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Gibco™ DMEM/F-12
GREENER_CHOICE

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Shipping Condition Room Temperature
Cell Line MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Content And Storage Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
Cell Type Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Sterilization Method Sterile-filtered
With Additives High Glucose,Glutamine,Phenol Red,Sodium Pyruvate
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification Animal Origin-free
Shelf Life 12 Months From Date of Manufacture
Product Line Gibco™
Serum Level Standard Serum Supplementation
2

Gibco™ SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red
GREENER_CHOICE

SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red

EDGE
3
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Gibco™ DMEM/F-12, GlutaMAX™ Supplement
GREENER_CHOICE

DMEM/F-12, GlutaMAX™ Supplement

Content And Storage 2 to 8°C
Description DMEM/F-12 Medium, 500mL, 1 X Concentration, Liquid Form, With GlutaMAX™, Phenol Red, Sodium Pyruvate, 6.8 to 7.2 pH, 12 Months Shelf Life, 290 to 330mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture
With Additives GlutaMAX,Phenol Red,Sodium Pyruvate
Product Type DMEM/F-12 Medium
Form Liquid
Without Additives No HEPES
pH 6.8 to 7.2
Certifications/Compliance ISO 13485 standards, FDA
Shelf Life 12 Months
Concentration 1 X
For Use With (Application) Mammalian cell culture
4

Cytiva HyClone™ DMEM/F12 1:1 Media

Used for culturing many different mammalian cells including MDCK, Glial cells, fibroblasts, and endothelial cells. HyClone™ DMEM/F12 1:1 Media supports cell culture growth and biomanufacturing processes and is manufactured using ISO 9001:2000-certified processes.

EDGE
Content And Storage 2° to 8°C, away from light
Sterilization Method 0.1 μm Sterile-filtered
Product Type DMEM/F12 1:1 Medium
Form Liquid
Certifications/Compliance ISO 9001:2000
Shelf Life 12 Months
Sterility Sterile-filtered
5
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Gibco™ DMEM/F-12, no glutamine
GREENER_CHOICE

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Form Liquid
Without Additives No Glutamine,No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Shelf Life 12 Months
6
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Gibco™ Advanced DMEM/F-12
GREENER_CHOICE

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Shipping Condition Room Temperature
Cell Line MRC-5, SP2, Vero, WI-38 and Jurkat
Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Without Additives No Glutamine,No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification Animal Origin
Shelf Life 12 Months From Date of Manufacture
Product Line Gibco™
Serum Level Reduced Serum
7
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Gibco™ DMEM/F-12, HEPES
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DMEM/F-12, HEPES

Regulatory Status For in-vitro diagnostic use
Content And Storage 2 to 8°C
Description DMEM/F-12 Medium, 500mL, 1 X Concentration, Liquid Form, With L-glutamine, HEPES, Phenol Red, Sodium Pyruvate, 6.9 to 7.3 pH, 12 Months Shelf Life, 270 to 330mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture
With Additives Glutamine,HEPES,Phenol Red,Sodium Pyruvate
Product Type DMEM/F-12 Medium
Form Liquid
pH 6.9 to 7.3
Certifications/Compliance ISO 13485 standards, FDA
Shelf Life 12 Months
Concentration 1 X
For Use With (Application) Mammalian cell culture
8
Promotions Available

Gibco™ DMEM/F-12, no phenol red
GREENER_CHOICE

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

9
Promotions Available

Gibco™ DMEM/F-12, GlutaMAX™ supplement
GREENER_CHOICE

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Sterilization Method Sterile-filtered
With Additives High Glucose,GlutaMAX,Phenol Red,Sodium Pyruvate
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Form Liquid
Without Additives No HEPES
Classification Animal Origin-free
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
10
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Gibco™ KnockOut™ DMEM/F-12
GREENER_CHOICE

KnockOut™ DMEM/F-12

11
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Gibco™ DMEM/F-12, no phenol red
GREENER_CHOICE

DMEM/F-12, no phenol red

12

Corning™ cellgro™ Basal Cell Culture Liquid Media - DMEM and Ham's F-12, 50/50 Mix
BIOPHARMA

Used for culture of epithelial, endothelial, and granulosa cell types under low serum conditions. Corning™ cellgro™ Basal Cell Culture Liquid Media - DMEM and Ham's F-12, 50/50 Mix has low levels of endotoxin, and absence of contamination.

13
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Gibco™ DMEM/F-12
GREENER_CHOICE

DMEM/F-12

14
Promotions Available

Gibco™ DMEM/F-12, HEPES, no phenol red
GREENER_CHOICE

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Cell Line MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Content And Storage Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Without Additives No Phenol Red,No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1X
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose,Glutamine,HEPES
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification Animal Origin-free
Shelf Life 12 Months From Date of Manufacture
Product Line Gibco™
Serum Level Standard Serum Supplementation
15

Corning™ cellgro™ DMEM/Ham's F-12 Mix
BIOPHARMA

Products undergo a variety of tests to determine functionality and toxicity using growth-promotion analysis. Corning™ cellgro™ DMEM/Ham's F-12 Mix support cell culture growth and substitution of Pyridoxine HCl for Pyridoxal ensures improved stability over original formulation.

Content And Storage 2 to 8°C
Product Type DMEM and Ham's F-12, 50/50 Mix
Form Liquid
Endotoxin Level <0.25EU/mL
Concentration 1 X
For Use With (Application) To assess growth promotion and cytotoxicity characteristics