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Gibco™ DMEM/F-12

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Brand:  Gibco™ 11320033

19.17 GBP valid until 2024-03-31
Use promo code "21883" to get your promotional price.



Code : C7

Additional Details : Weight : 0.59000kg

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Product Code. 11554546

  • £22.08 / 500mL
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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES

The complete formulation is available.

Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications

Specifications

Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
1 X
DMEM (Dulbecco's Modified Eagle Medium)/F-12
Sterile-filtered
No HEPES
cGMP-compliant under the ISO 13485 standard
500 mL
MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Animal Origin-free
Liquid
Standard Serum Supplementation
High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Sustainable packaging
Gibco™
Room Temperature
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.