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Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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1 – 15 of 127 results
1

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

EDGE
2

Cytiva PD MidiTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 1mL. Cytiva Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

3

Cytiva Ni Sepharose™ excel

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

Particle Size 90μm
Buffer 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
Flow Rate 150 to 600cm/hr.
Content And Storage 4° to 30°C
pH Range 2 to 12
Product Type Ni Sepharose excel chromato graphy media
dimMatrix Ni-Sepharose
Support Type 6% Highly Cross-linked Agarose
4

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
5

Cytiva His GraviTrap™ Columns

Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.

EDGE
6

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

EDGE
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose
7

Cytiva HiTrap™/HiPrep™ Column Accessories

EDGE
Column Type Column Accesory
Material Polypropylene
8

Cytiva StrepTrap XT Chromatography Column

StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.

EDGE
Height (Metric) 25 mm
Column Type Affinity Column
For Use With (Application) Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™
9

Cytiva Superose™ 6 Increase Columns

High-resolution size-exclusion chromatography for studying large proteins and complexes. Cytiva Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).

EDGE
Particle Size 8.6μm
Ligand Type Cross-linked agarose
Content And Storage 4° to 30° in 20% ethanol
pH Range 3 to 12
Column Type Gel Filtration Column
Material Borosilicate glass
Max. Pressure 435 psi
10

Cytiva HisTrap™ FF Crude Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Particle Size 45 to 165 μm
Type Pre-packed Column
Content And Storage 4°C to 30°C
pH Range 3 to 12
Material Polypropylene
Support Type 6% Cross-linked Agarose
11

Cytiva HiScreen™ Capto™ Q ImpRes and Capto™ SP ImpRes

High-resolution intermediate purification and polishing based on the well-established Capto platform with traditional ligands

EDGE
Particle Size 36 to 44μm
Buffer 1M NaOH, 8M Urea, 6M Guanidine Hydrochloride, 30% Isopropanol and 70% Ethanol
Flow Rate 220 cm/hr.
Height (Metric) 200mm
Content And Storage 4° to 30°C
Diameter (English) Inner 39.3 in.
Diameter (Metric) Inner 1000mm
dimMatrix Capto Q Chromatography Media
Quantity 100 mL
For Use With (Application) High-throughput intermediate purification and polishing steps of a wide range of biomolecules
Support Type High-flow Agarose
Max. Pressure <3 bar
Height (English) 7.87 in.
12

Cytiva Thrombin Protease

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

EDGE
13

Cytiva HisTrap™ excel Prepacked Columns

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

EDGE
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 2 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
14

Cytiva GSTrap™ FF Columns: 16 x 25mm

Purifies GST-tagged proteins in one step

EDGE
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.62 in.
Bed Volume 5 mL
Description GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
For Use With (Application) For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract
Particle Size 90 μm
Ligand Type Glutathione and 10 carbon linker arm
Flow Rate <20 mL/min.
Type Pre-packed Column
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Support Type 4% Highly Cross-linked Agarose
15

Cytiva Protein G HP SpinTrap™ Columns

For small-scale purification of antibodies or enrichment of proteins of interest in prepacked spin columns