Protein Analysis Reagents
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Thermo Scientific™ Pierce™ BCA Protein Assay Kits
The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.
| Assay | BCA Assay |
|---|---|
| For Use With (Equipment) | Spectrophotometer, Microplate Reader |
| Product Type | Protein Quantitation Assay |
| For Use With (Application) | Solution-based Detection, Absorbance |
| Product Line | Pierce |
| Detection Method | Colorimetric |
Thermo Scientific™ Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Perform high-throughput protein recovery (7K MWCO) and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Thermo Scientific Pierce Zeba desalting chromatography columns, plates, and cartridges.
| MWCO | 7.0 kDa |
|---|---|
| Column Type | Size-exclusion, Proprietary Resin |
| Purification Target | Buffer Exchange, Protein |
| Product Line | Zeba |
Thermo Scientific™ RIPA Lysis and Extraction Buffer
Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.
| Content And Storage | Upon receipt store at 4°C. |
|---|---|
| Format | Liquid |
| Product Type | Extraction Buffer |
Thermo Scientific™ Lysozyme Solution (50 mg/mL)
Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.
Invitrogen™ Dynabeads™ Protein G for Immunoprecipitation
Dynabeads Protein G beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein G (∼17 kDa) covalently coupled to the surface.
| Shipping Condition | Ambient temperature |
|---|---|
| Content And Storage | Dynabeads Protein G are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C. |
| Purity or Quality Grade | For Research Use Only |
| Description | Recombinant Protein G covalently bound to Dynabeads |
| Surface Functionality | Protein G |
| Material | Polystyrene |
| Concentration | 30 mg/mL |
| For Use With (Application) | Immunoprecipitation |
| High-throughput Compatibility | High-throughput Compatible |
| Ligand Type | Protein G |
| Type | Superparamagnetic Beads |
| Target | Antibodies |
| Format | Beads in suspension |
| For Use With (Equipment) | KingFisher™ Sample Purification System, DynaMag™ magnets |
| Diameter (Metric) | 2.8 μm |
| Certifications/Compliance | ISO9001 and ISO13485 |
| Uniformity | Monosized 2.8 mm (CV <5%) |
| Product Line | Dynabeads |
Cytiva™ Disposable PD-10 Desalting Columns
Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. Cytiva Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.
Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)
Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.
| Buffer | Lysis Buffer |
|---|---|
| Content And Storage | Upon receipt store at 4°C |
| Inhibitors | Protease Inhibitor |
| Form | Liquid |
| Product Type | Protease Inhibitor Cocktail |
| Reagent Type | Protease Inhibitor |
| Product Line | Halt |
Invitrogen™ Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 488 is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Store in freezer (-5 to -30°C) and protect from light. |
| Product Type | Dye |
| Label or Dye | Alexa Fluor™ 488 |
| Emission | 517 nm |
| Excitation | 494 nm |
| Label Type | Alexa Fluor |
| Chemical Reactivity | Amine |
| Product Line | Alexa Fluor |
Invitrogen™ Qubit™ Protein and Protein Broad Range (BR) Assay Kits
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Achieve sensitive detection of protein, and low protein-to-protein variation, with the Qubit Protein and Protein BR Assay Kits for fluorescence-based protein quantitation assays.
| Product Line | Qubit |
|---|---|
| Detection Method | Fluorescence |
Invitrogen™ Dynabeads™ M-270 Epoxy
Dynabeads™ M-270 Epoxy beads are 2.8 μm superparamagnetic beads containing surface epoxy groups.
| Shipping Condition | Room Temperature |
|---|---|
| Type | Epoxy Bead |
| Purity or Quality Grade | Research Grade |
| Format | Freeze Dried |
| Surface Functionality | Epoxy |
| Sample Type | Any Sample Type |
| Diameter (Metric) | 2.8 μm |
| Shelf Life | 24 months from date of manufacture |
| Elution Volume Min | 5 μL |
| Product Line | DYNAL, Dynabeads |
Thermo Scientific™ Mass Spectrometry Grade Proteases
Improve sequence coverage and protein identification for mass spectrometry with high-quality, MS-grade proteases provided in stable liquid or lyophilized formats. Suitable for either in-solution or in-gel digestion workflows, each protease may be used individually or in combination to improve protein sequence coverage.
| Workflow Step | Protein Digestion |
|---|---|
| For Use With (Equipment) | Mass Spectrometer |
| Grade | MS |
| Detection Method | Mass Spectrometry |
| Product Line | Pierce |
Thermo Scientific™ SnakeSkin™ Dialysis Tubing
Perform desalting and buffer exchange for 10 to 100 mL samples with this 3.5K, 7K, and 10K molecular weight cut-off (MWCO), ready-to-use form of traditional dialysis membrane tubing. Securely close dialysis tubing up to 50 mm flat diameter with the sturdy, non-floating tube SnakeSkin Dialysis Clips.
| Product Line | SnakeSkin |
|---|
Thermo Scientific™ Pierce™ 660nm Protein Assay Reagent
Red-to-green (A660nm), ready-to-use, detergent- and reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.
Thermo Scientific™ Pierce™ Premium Grade DSP (dithiobis(succinimidyl propionate))
Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this popular amine-reactive, cleavable crosslinker.
| Shipping Condition | Ambient |
|---|---|
| Content And Storage | Upon receipt store desiccated at 4°C. |
| Cleavable | By Thiols |
| Water Soluble | No |
| Form | Powder |
| Molecular Weight (g/mol) | 404.42 |
| Solubility | DMF, DMSO |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Homobifunctional |
| Chemical Reactivity | Amine-Amine |
| Cell Permeability | Yes |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Medium (10 to 30 Å) |
| Formulation | Dithiobis(succinimidylpropionate); powder solid |
| Reactive Moiety | NHS Ester |
| Spacer Arm Length | 12.0 Å |
| Product Line | Pierce |
| PEGylated | No |
Invitrogen™ Dynabeads™ Protein A for Immunoprecipitation
Dynabeads Protein A beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the surface.
| Shipping Condition | Ambient temperature |
|---|---|
| Content And Storage | Dynabeads Protein A are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C. |
| Purity or Quality Grade | For Research Use Only |
| Description | Recombinant Protein A covalently bound to Dynabeads |
| Surface Functionality | Protein A |
| Material | Polystyrene |
| Concentration | 30 mg/mL |
| For Use With (Application) | Immunoprecipitation |
| High-throughput Compatibility | High-throughput Compatible |
| Ligand Type | Protein A |
| Type | Superparamagnetic Beads |
| Target | Antibodies |
| Format | Beads in suspension |
| For Use With (Equipment) | KingFisher™ Sample Purification System, DynaMag™ magnets |
| Diameter (Metric) | 2.8 μm |
| Certifications/Compliance | ISO9001 and ISO13485 |
| Uniformity | Monosized 2.8 mm (CV <5%) |
| Product Line | Dynabeads |