Bioassays, kits, and reagents used to analyze various properties of living cell samples. Products include apoptosis assays, signal transduction assays, and cell growth and differentiation assays.
Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells, the non-fluorescent Calcein AM is converted to green or blue fluorescent Calcein, after acetoxymethyl ester hydrolysis by intracellular esterases.
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The LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining, or prior to elimination of biohazardous materials using formaldehyde fixation.
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Invitrogen CellMask™ plasma membrane stains enable fast and uniform labeling of the plasma membrane in almost all cell types. These stains work rapidly and can be detected using standard microscope filter sets with any imaging instrument.
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SYTO Green Fluorescent Nucleic Acid Stains exhibit bright, green fluorescence upon binding to nucleic acids. These dyes are cell-permeant and can be used to stain RNA and DNA in live cells, dead cells, and bacteria.
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Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix.
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CyQUANT cell proliferation assays are fast and highly sensitive fluorescence methods for counting cells to assess cell growth and cytotoxicity in microplate format. These assays use fluorescent nucleic-acid-binding dyes to measure cell number based on DNA content.
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The Amplex Red and UltraRed reagents are fluorogenic substrates for peroxidase. These highly sensitive and stable probes react with H2O2 in the presence of HRP for solution-based assays and ELISAs. Amplex UltraRed is a brighter version of Amplex Red with an improved pH range and sensitivity.
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MitoSOX superoxide indicators are novel fluorogenic dyes specifically targeted to mitochondria in live cells. Oxidation of the MitoSOX reagent by mitochondrial superoxide produces bright green or red fluorescence.
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The LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining, or prior to elimination of biohazardous materials using formaldehyde fixation.
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Healthy mitochondrial membranes maintain a difference in electrical potential between the interior and exterior of the organelle, referred to as a membrane potential.
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Assess cell viability with Dead Cell Apoptosis kits with Annexin V and conjugated fluorescent dyes such as Alexa Fluor 488, FITC, PI, PE, APC, and SYTOX Green.
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Contains 1 vial of annexin V, FITC conjugate (250 μL ), 1 vial of propidium iodide (PI, 100 μL), and 1 bottle of annexin binding buffer (5X solution, 15 mL). Store in refrigerator (2°C to 8°C) and protect from light.