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Hot Start PCR Reagents and Kits

In hot start PCR, key reagents are prevented from reacting in the PCR mixture until optimal temperatures are met. This reduces non-specific DNA amplification that occurs naturally at lower temperatures, increasing product yield, specificity, and sensitivity.
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Promotions Available

DreamTaq™ Hot Start PCR Master Mixes

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.

EDGE
Shipping Condition Dry Ice
Polymerase DreamTaq DNA Polymerase
GC-Rich PCR Performance Low
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 2X
For Use With (Application) Hot-start PCR
2
Promotions Available

Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)

Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.

EDGE
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Format Tube
Size (Final Product) 5 kb or less
Hot Start Built-In Hot Start
Concentration 2X
For Use With (Application) Hot-start PCR
3
Promotions Available

Invitrogen™ Platinum™ Direct PCR Universal Master Mix
GREENER_CHOICE

Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.

EDGE
Shipping Condition Dry Ice
Polymerase Platinum II Taq Hot-Start DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Size (Final Product) 8 kb
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Universal Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 2X
For Use With (Application) Hot-start PCR,Real Time PCR (qPCR)
4
Promotions Available

Thermo Scientific™ Phire Hot Start II PCR Master Mixes

Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.

EDGE
Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 2X
Reaction Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
Starting Material DNA
For Use With (Application) Cloning,Hot-start PCR
5
Promotions Available

Applied Biosystems™ AmpliTaq Gold™ Fast PCR Master Mix

The Applied Biosystems AmpliTaq Gold Fast PCR Master Mix is a premix, hot start chemistry for end-point PCR analysis.

EDGE
Shipping Condition Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Overhang 3'-A
Concentration 2X
For Use With (Application) Cloning,Hot-start PCR
Exonuclease Activity 5' - 3'
Polymerase AmpliTaq Gold DNA Polymerase
Size (Final Product) 1.5 kb or less
Reaction Format SuperMix or Master Mix
Product Type Fast PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
6
Promotions Available

Invitrogen™ Platinum™ Hot Start PCR Master Mixes (2X)

Invitrogen Platinum Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.

Shipping Condition Dry Ice
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 1X
Format Tube
Size (Final Product) 5 kb or less
Reaction Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 2X
Starting Material DNA
For Use With (Application) Hot-start PCR
7
Promotions Available

Applied Biosystems™ Platinum™ Multiplex PCR Master Mix

The Platinum Multiplex PCR Master Mix is designed specifically for endpoint multiplex PCR.

Shipping Condition Dry Ice
Polymerase Platinum Taq DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Size (Final Product) 2.5 kb or less
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Concentration 2X
For Use With (Application) Hot-start PCR,Multiplex PCR
Multiplex Capability Up to 20-plex
8
Promotions Available

Invitrogen™ PCR Master Mix Starter Pack

The PCR Master Mix Starter Pack is composed of three different PCR master mixes for easy evaluation of their suitability for use in your research.

9
Promotions Available

Applied Biosystems™ True Allele™ PCR Premix

Applied Biosystems True Allele PCR Premix is a PCR master mix that contains AmpliTaq Gold DNA Polymerase, dNTPs, MgCl2, and buffer.

10
Promotions Available

Invitrogen™ Platinum™ II Green PCR Buffer (5X)

Invitrogen 5X Platinum II Green PCR Buffer for fast and easy PCR

11

Macherey-Nagel™ NucleoType Seed PCR, DirectPCR kit for genotyping of seed

Time and money saving seed genotyping workflow.

Content And Storage Lysis Buffer P; Proteinase K; NucleoType HotStart PCR Master Mix (2x) containing polymerase, dNTPs, buffer, enhancer, loading dye and stabilizer. Store at 4°C or -20°C
Format Kit
Size (Final Product) Amplicon size up to 2000 bp
Sample Type Hard plant material like e.g., seeds from soybeen, wheat, corn, rice, as well as from moss, fern leaf, and fir needle
Reaction Speed 30 to 90 min.
Kit Type Simple sample preparation suitable for hot start PCR
For Use With (Application) Genotyping
12

Macherey-Nagel™ NucleoType Mouse PCR, DirectPCR kit for genotyping of mouse samples

Kit for rapid mouse typing experiments with common samples such as tail clips, ear punch, hair, and blood.

Activation Time Lysis 2 min; Enzyme inactivation 3 min; PCR cycling 30–90 min
Content And Storage NucleoType HotStart PCR Master Mix (2x), Lysis Buffer M, Proteinase K. Store at 4 °C or -20 °C
Format Kit
Size (Final Product) Amplicon size: up to 1000 bp
Sample Type Mouse tail clipping (1 mm), mouse ear punch (Dia. 1 mm), mouse blood (1 μL), mouse hairs (approx. 3–30)
Yield 100 - 600 ng amplicon per 10 μL PCR
Shelf Life 1 year
For Use With (Application) Genotyping
Detection Method Primer-probe
13

Abnova™ 2 x HotTaq PCR Mix with MgCl2

HotTaq PCR mix is a prepared solution containing everything needed for successful PCR reaction except specific primers and DNA template.

14
Promotions Available

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

EDGE
Polymerase AmpliTaq Gold DNA Polymerase
Fidelity (vs. Taq) 1X
Size (Final Product) 5 kb or less
Product Type DNA Polymerase
Overhang 3'-A
Concentration 5 U/μL
Exonuclease Activity 5' - 3'
15
Promotions Available

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

EDGE
Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 5 U/μL
Exonuclease Activity 5' - 3'