Invitrogen™ Platinum™ Direct PCR Universal Master Mix

Product Code. 17118430

Brand: Invitrogen™ A44647100

Description

Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.

Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA. It contains high-performing, engineered Platinum II Taq Hot-Start DNA Polymerase with dNTPs in an innovative buffer that enables universal primer annealing for superior performance in direct PCR applications.

Features of Platinum Direct PCR Universal Master Mix include:

• Direct DNA amplification from various samples
• Universal primer annealing at 60°C
• Optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets
• Superior specificity, sensitivity, and yields

Platinum Direct PCR Universal Master Mix is designed to work with a variety of samples of different origins such as animal, human, plant, insect, worm, and bacterial samples. The quick lysis protocol enables efficient amplification from templates up to 8 kb in size and co-cycling of different length fragments.

Engineered Platinum II Taq DNA polymerase confers faster cycling and tolerance to reaction inhibitors originating from sample material. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step. This automatic 'hot start' provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.

Due to the unique composition of the Platinum Direct PCR Universal Master Mix, the annealing temperature of 60°C can be used for most primer pairs that follow the general design rules. Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. Different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.

Specifications

• Concentration: 2X
• Content And Storage: • 2X Platinum Direct PCR Universal Master Mix (1 mL)
  • Lysis Buffer (5 mL)
  • Proteinase K (150 μL)
  • Platinum GC Enhancer (1.25 mL)
  • Nuclease-free water (1.25 mL)
  
  Store at –20°C.
• GC-Rich PCR Performance: High
• Polymerase: Platinum II Taq Hot-Start DNA Polymerase
• Reaction Speed: Fast
• Green Features: Less hazardous, less waste
• Product Type: Direct PCR Universal Master Mix
• Quantity: 100 reactions
• Shipping Condition: Dry Ice
• For Use With (Application): Hot-start PCR, Real Time PCR (qPCR)
• Fidelity (vs. Taq): 1X
• Hot Start: Built-In Hot Start
• No. of Reactions: 100 Reactions
• Overhang: 3'-A
• Reaction Format: SuperMix or Master Mix
• Size (Final Product): 8 kb