Purity or Quality Grade |
Affinity chromatography |
Conjugate |
Unconjugated |
Specific Reactivity |
Mouse |
Form |
Liquid |
pH Range |
8.0 |
Common Name |
Mouse HMGB1 Carrier-Free (High Mobility Group Box 1) |
Molecular Weight (g/mol) |
The protein contains an N-terminal His tag and has a predicted molecular mass of 27.6kDa. |
Endotoxin Concentration |
Less than 0.01ng/μg cytokine as determined by the LAL assay. |
For Use With (Application) |
Control,Functional Assay |
Source |
E. coli |
Name |
Mouse HMGB1 Carrier-Free (High Mobility Group Box 1) |
Purification Method |
SDS-PAGE |
Biological Activity |
The ED50 of this protein, as measured by mouse TNF alpha induction in RAW264.7 cells, is 3-25μg/mL in the presence of 10μg/mL Polymyxin B. This corresponds to a specific activity of 3,300-40 Units/mg. |
Formulation |
20mM tris HCl with 0.2M NaCl, 1mM DTT and no preservative; pH 8.0 |
Species |
E. coli |
Recombinant |
Recombinant |
Gene Symbol |
Hmgb1 |
Storage Requirements |
-80°C |
Concentration |
0.5mg/mL |
Protein Subtype |
Recombinant |
Accession Number |
P63158 |
Regulatory Status |
RUO |
Product Type |
Recombinant Protein, Carrier-Free |
Gene ID (Entrez) |
15289 |
Structural Form |
E. coli expressed amino acids Met1-Glu215 of mouse HMGB-1 accession # NM_010439 |
Shelf Life |
12 Months |
Cross Reactivity |
Ms |