ZO-1, clone: R26.4C, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9776-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: The monoclonal antibody R26.4C recognizes the tight junction protein 1, ZO-1 (zona occludens) of rat, mouse, canine, and porcine. ZO-1 is a member of the MAGUK family of tight junction associated scaffolding proteins, each containing a PDZ, SH3 and guanylate kinase-like (GUK) domain. ZO-1 is an integral member of the tight junction complex which forms a barrier to paracellular movement of substances, separating apical and basolateral fluids in relation to the epithelial cell layer. ZO-1 forms complexes with family members ZO-2, ZO-3, as well as occludin, cingulin, claudin-1-8, actin, alpha-catenin, the Ras-effector molecule AF-6, ZONAB, Apg-2, and the junctional adhesion molecules (JAM)-A-C. ZO-1 is thought to anchor the actin cytoskeleton to the tight junction. ZO-1 and ZO-2 have been shown to regulate cell cycle and proliferation with recent evidence highlighting the down-regulation of ZO-1 and ZO-2 with cancer progression.Applications Reported: This R26.4C antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, microscopy, and immunocytochemistry.Applications Tested: This R26.4C antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells and by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|DKFZp686M05161, MGC133289, ZO-1|
|ImmunocytochemistryþImmunohistochemistry (Frozen)þMicroscopyþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.