ZAP70 Antibody (SPM362) - IHC-Prediluted, Novus Biologicals™

Mouse Monoclonal Antibody

Brand:  Novus Biologicals NBP2-48250

Product Code. 18147538

Description

ZAP70 Monoclonal specifically detects ZAP70 in Human samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: ZAP70
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: 1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Symbols: ZAP70
• Immunogen: Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70 (Uniprot: P43403)
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
• Content And Storage: Store at 4C.
• Isotype: IgG2a κ

• Applications: Immunohistochemistry (Paraffin)
• Clone: SPM362
• Dilution: Immunohistochemistry-Paraffin
• Gene Alias: 70 kDa zeta-associated protein, EC 2.7.10, EC 2.7.10.2, SRKFLJ17679, STDFLJ17670, Syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, TZK, ZAP-70, zeta-chain (TCR) associated protein kinase (70 kD), zeta-chain (TCR) associated protein kinase 70kDa, zeta-chain associated protein kinase, 70kD
• Host Species: Mouse
• Molecular Weight of Antigen: 70 kDa
• Quantity: 7 mL
• Research Discipline: Adaptive Immunity, Cell Biology, Immunology, Protein Kinase, Signal Transduction, Tyrosine Kinases
• Gene ID (Entrez): 7535
• Target Species: Human
• Form: Purified