Invitrogen™ Human ZAP-70, His Tag Recombinant Protein

Product Code. 15418383

Brand: Invitrogen™ PR3017C

Description

Recombinant Protein

Protein Form: Full Length, Recombinant, Cytoplasmic Assay Entry: Biochemical phospho-specific activity (no antibody), Biochemical terbium phospho-specific activity, Biochemical europium phospho-specific activity, Biochemical binding/kinetic System Compatibility: LanthaScreen™ Antibody, LanthaScreen™ Substrate, Z'-LYTE™, Omnia™ Bulk Substrate

ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.

Specifications

• Accession Number: P43403
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 25 mM HEPES with 0.5 mM DTT, 150 mM NaCl, 50% glycerol and no preservative; pH 7.5
• Gene ID (Entrez): 7535
• Molecular Weight (g/mol): 70.1 kDa
• Name: Human ZAP-70, His Tag
• Quantity: 100 μg
• Regulatory Status: RUO - research use only
• Gene Alias: 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; EC 2.7.10.2; FLJ17670; FLJ17679; I79_004087; IMD48; kinase ZAP70; mrtle; mur; Selective T cell defect; Srk; STCD; STD; syk-related protein tyrosine kinase; syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; TZK; ZA70; Zap70; ZAP-70; Zeta chain associated protein kinase 70 kDa; zeta chain of T cell receptor associated protein kinase; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70 kDa; zeta-chain (TCR) associated protein kinase; zeta-chain (TCR) associated protein kinase 70; zeta-chain (TCR) associated protein kinase 70 kDa; zeta-chain associated protein kinase 70 kDa; zeta-chain associated protein kinase, 70 kD
• Common Name: Zap-70
• KinaseFamily: SYK Kinase Family
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Full length
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: Baculovirus
• Form: Liquid