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Zap-70 Polyclonal Antibody, Invitrogen™
Description
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
Mutations in ZAP-70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP-70 expression also defines a subset of Chronic Lymphocytic Leukaemia (CLL) in patients with unmutated Ig gene and poor clinical course.
Specifications
Specifications
| Antigen | ZAP-70 |
| Applications | Immunohistochemistry (Paraffin), Western Blot |
| Classification | Polyclonal |
| Conjugate | Unconjugated |
| Gene | ZAP70 |
| Gene Accession No. | P43403 |
| Gene Alias | SRK, STD, TZK, ZAP-70, 70 kDa zeta-associated protein, 70 kDa zeta-chain associated protein, syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, zeta-chain associated protein kinase, 70kD |
| Gene Symbols | ZAP70 |
| Host Species | Rabbit |
| Immunogen | Synthetic peptide corresponding to C-terminus of human ZAP-70. |
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