Invitrogen™ Zap-70 Monoclonal Antibody (1E7.2), Pacific Blue™

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MHZAP7028

Product Code. 10287154

Description

Our Pacific Blue™ dye is optimally excited by the violet laser and conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.

ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.

Specifications

• Antigen: Zap-70
• Classification: Monoclonal
• Conjugate: Pacific Blue
• Gene: ZAP70
• Gene Alias: 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; EC 2.7.10.2; FLJ17670; FLJ17679; I79_004087; IMD48; kinase ZAP70; mrtle; mur; Selective T cell defect; Srk; STCD; STD; syk-related protein tyrosine kinase; syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; TZK; ZA70; Zap70; ZAP-70; Zeta chain associated protein kinase 70kDa; zeta chain of T cell receptor associated protein kinase; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase; zeta-chain (TCR) associated protein kinase 70; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: ASR
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry
• Clone: 1E7.2
• Formulation: PBS with 4mg/mL BSA and 0.1% sodium azide
• Gene Accession No.: P43403, P43404
• Gene Symbols: ZAP70
• Immunogen: GST-fusion protein of Human ZAP70, amino acids 282-307.
• Quantity: 500 μL
• Research Discipline: Signal Transduction
• Gene ID (Entrez): 22637, 7535
• Content And Storage: 4°C, store in dark
• Form: Liquid