Invitrogen™ Zap-70 Monoclonal Antibody (1E7.2)

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MA121350

Product Code. 11593923

Description

Positive control of Jurkat cell lysate suggested.

ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.

Specifications

• Antigen: Zap-70
• Classification: Monoclonal
• Concentration: 1.7 mg/mL
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene Accession No.: P43403, P43404
• Gene Symbols: ZAP70
• Immunogen: Peptide conjugated to KLH, corresponding to amino acids 282-307 of human ZAP-70.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Flow Cytometry, Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 1E7.2
• Conjugate: Unconjugated
• Gene: ZAP70
• Gene Alias: 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; EC 2.7.10.2; FLJ17670; FLJ17679; I79_004087; IMD48; kinase ZAP70; mrtle; mur; Selective T cell defect; Srk; STCD; STD; syk-related protein tyrosine kinase; syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; TZK; ZA70; Zap70; ZAP-70; Zeta chain associated protein kinase 70kDa; zeta chain of T cell receptor associated protein kinase; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase; zeta-chain (TCR) associated protein kinase 70; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 22637, 301348, 7535
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid