Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 17 Peptide

Product Code.	Quantity	unitSize
10328882	800 x 20μL, 800 x 20 μL Assays	16000µL

Product Code. 10328882

Brand: Thermo Scientific™ PV4316

Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase, containing Ser-7.

Specifications

• Description: Z'-LYTE™ Ser/Thr 17 Peptide Kinase Assay Kit, 800 x 20μL, 800rxns, Serine/Threonine Kinase Assay Type, Peptide-Based Substrate, 535nm Emission, 405/465nm Excitation, Methylcoumarin Label, End Point Readout, Serine/Threonine Kinases Target Enzyme, FRET Technique, Used for Pharma and Biopharma, Protein Assays and Analysis
• Product Line: Z ́-LYTE
• For Use With (Application): Pharma and biopharma, protein biology, drug discovery and development, target and lead identification and validation, kinase biology, biochemical kinase assays, z and acute-lyte™ kinase assays, enzyme and protein activity assays, protein assays and, FRET
• Includes: Z'-LYTE™ Ser/Thr 11 peptide, Z'-LYTE™ Ser/Thr 11 phospho-peptide, 5X kinase buffer A, ATP, development reagent B, development buffer
• Sample Volume: 800 x 20μL
• Assay: Serine/Threonine Kinase Assay
• Assay Entry: Biochemical phospho-specific activity (no antibody)
• Content And Storage: –80°C, Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
• Product Type: Z'-LYTE™ Ser/Thr 17 Peptide Kinase Assay Kit
• Quantity: 800 x 20μL, 800 x 20 μL Assays
• Format: 384-well plate
• Excitation/Emission: 405/465, 535 nm
• Label or Dye: Methylcoumarin
• No. of Reactions: 800 Reactions
• Readout: End Point
• References: Biochem J. vol 296, pp. 843-9 (1993)
• Shipping Condition: Dry Ice
• Source: Similar to Ser-7 of Glycogen Synthase KKLNRTLSVA (KKLNRTLSFAEPG)
• Substrate Properties: Peptide-Based Substrate
• Target Entry: CAMK2B, PRKCN, PRKD2, DCAMKL2, PRKD1, NEK4, MELK
• Target Enzyme: Serine/Threonine Kinases
• Validated Kinases: CAMK2B, DCAMKL2 , MELK, NEK4, PRKCN , PRKD1, PRKD2

For Research Use Only. Not for use in diagnostic procedures.