Y-27632 dihydrochloride, Tocris Bioscience™

Product Code	Brand	Quantity
30001217	Tocris Bioscience 1254/1	1 mg
18084362	Tocris Bioscience 1254/10	10 mg
18044982	Tocris Bioscience 1254/50	50 mg

Description

Y-27632 dihydrochloride is a selective ROCK inhibitor (Ki values are 0.14-0.22, 0.3, 25, 26 and > 250 μM for ROCK1 (p160 ROCK), ROCK2, PKA, PKC and MLCK respectively). Also inhibits PRK2 (IC50 = 600 nM). Increases survival rate of human embryonic stem (hES) cells and iPSC undergoing cryopreservation. Used in combination with CHIR99021, RepSox, Forskolin, SP 600125, Go 6983 and VPA to reprogram fibroblasts to mature neurons. Also optimizes naive human pluripotent stem cell growth and viability following naive cell derivation from primed ESCs and iPSCs using naive human stem cell medium (NHSM). Used as component of growth media for urothelial organoids. Cell-permeable, orally bioavailable and active in vivo. Y-27632 synthesized to cGMP guidelines also available.

For more information about how Y-27632 dihydrochloride may be used, see our protocols: Transdifferentiating Fibroblasts into Neurons, Converting Fibroblasts into Cardiomyocytes (9C Cocktail), Accelerated Induction of Cortical Neurons from hiPSCs, Human iPSC-derived Myoblasts .

Chemical Identifiers

• CAS: 129830-38-2
• Molecular Weight (g/mol): 320.258
• Synonym: y-27632 dihydrochloride, y 27632 dihydrochloride, unii-q9828ii7f3, 1r,4r-4-r-1-aminoethyl-n-pyridin-4-yl cyclohexanecarboxamide dihydrochloride, 1r,4r-4-r-1-aminoethyl-n-pyridin-4-yl cyclohexane-1-carboxamide dihydrochloride, y 27632 hydrochloride, 4-1r-1-aminoethyl-n-pyridin-4-yl cyclohexane-1-carboxamide dihydrochloride, 1r,4r-4-1r-1-aminoethyl-n-pyridin-4-yl cyclohexane-1-carboxamide dihydrochloride, r-+-trans-4-1-aminoethyl-n-4-pyridyl cyclohexanecarboxamide dihydrochloride
• IUPAC Name: 4-[(1R)-1-aminoethyl]-N-pyridin-4-ylcyclohexane-1-carboxamide;dihydrochloride

• Molecular Formula: C14H23Cl2N3O
• InChI Key: IDDDVXIUIXWAGJ-DDSAHXNVSA-N
• PubChem CID: 9901617
• SMILES: CC(C1CCC(CC1)C(=O)NC2=CC=NC=C2)N.Cl.Cl

Specifications

• CAS: 129830-38-2
• Synonym: y-27632 dihydrochloride, y 27632 dihydrochloride, unii-q9828ii7f3, 1r,4r-4-r-1-aminoethyl-n-pyridin-4-yl cyclohexanecarboxamide dihydrochloride, 1r,4r-4-r-1-aminoethyl-n-pyridin-4-yl cyclohexane-1-carboxamide dihydrochloride, y 27632 hydrochloride, 4-1r-1-aminoethyl-n-pyridin-4-yl cyclohexane-1-carboxamide dihydrochloride, 1r,4r-4-1r-1-aminoethyl-n-pyridin-4-yl cyclohexane-1-carboxamide dihydrochloride, r-+-trans-4-1-aminoethyl-n-4-pyridyl cyclohexanecarboxamide dihydrochloride
• SMILES: CC(C1CCC(CC1)C(=O)NC2=CC=NC=C2)N.Cl.Cl
• Molecular Weight (g/mol): 320.258
• Formula Weight: 320.26
• Chemical Name or Material: Y-27632 dihydrochloride

• Molecular Formula: C14H23Cl2N3O
• InChI Key: IDDDVXIUIXWAGJ-DDSAHXNVSA-N
• IUPAC Name: 4-[(1R)-1-aminoethyl]-N-pyridin-4-ylcyclohexane-1-carboxamide;dihydrochloride
• PubChem CID: 9901617
• Percent Purity: >98%

Safety and Handling

Recommended Storage : Desiccate at Room Temperature