Invitrogen™ Mouse M-CSF (CSF1) ELISA Development Kit (ABTS), PeproTech®

Product Code. 30087907

Brand: Invitrogen™ 900K245K

Description

ELISA

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.

Specifications

• Accession Number: P07141
• Assay Range: Lot-specific
• Assay Sensitivity: Lot-specific
• Conjugate: HRP
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 12977
• Gene Symbol: Csf1
• Kit Contents: Capture Antibody, Detection Antibody, Protein Standard, HRP-conjugate
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: M-CSF
• Test Time: 1 hrs 45 mins
• Total Assay Time: 24 hrs 30 mins