WTAP Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA527764

Product Code. 13491917

Description

Recommended positive controls: Jurkat nuclear extract, K562 nuclear extract. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (90%), Xenopus laevis (92%), Pig (100%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and is observed in both sporadic and inherited forms. The Wilms' tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators, and functions as a transcriptional repressor. WTAP (Wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may be involved in regulating mRNA splicing. WTAP is found in nuclear speckles, where it regulates the G2/M cell cycle transition by binding to the 3' UTR of cyclin A2, thus enhancing its stability. Additionally, WTAP inhibits expression of WT1 target genes and is able to impair the ability of WT1 to bind DNA. Two isoforms of WTAP exist due to alternative splicing events.

Specifications

• Antigen: WTAP
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: WTAP
• Gene Alias: 2810408K05Rik; 9430038B09Rik; Female-lethal(2)D homolog; hFL(2)D; KIAA0105; Mum2; PNAS-132; pre-mRNA-splicing regulator WTAP; putative pre-mRNA splicing regulator female-lethal(2D); RGD1563824; Wilms tumor 1 associated protein; wilms tumor 1-associating protein; Wilms' tumour 1-associating protein; Wilms tumour 1-associating protein; WT1-associated protein; WTAP
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 9589
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q15007
• Gene Symbols: WTAP
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 215 of Human WTAP
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG