WT1 Antibody (WT1/857 + 6F-H2) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
18139537	Novus Biologicals NBP2-47858-0.1MG	0.1 mg

Description

WT1 Monoclonal specifically detects WT1 in Human, Mouse, Rat samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin, CyTOF-ready.

Specifications

• Antigen: WT1
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Angiogenesis, Cancer, Cell Cycle and Replication, Cellular Markers, Chromatin Research, Tumor Suppressors
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 7490
• Immunogen: Recombinant full length human WT1 protein (WT1/857); Recombinant fragment aa1-181 of human WT1 (6F-H2)
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: WT1/857 + 6F-H2
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat
• Gene Alias: AWT1, NPHS4GUD, WAGR, Wilms tumor 1, Wilms tumor protein, WIT-2, WT33
• Gene Symbols: WT1
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors.WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.