Invitrogen™ VWF Human ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15475173	Invitrogen™ EHVWF	96 Tests
17185599	Invitrogen™ EHVWFX5	5 x 96 Tests

Description

The Human von Willebrand Factor (vWF) ELISA quantitates Hu vWF in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu vWF. Principle of the method The Human vWF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Factor VIII related antigen or von Willebrand factor is a multimeric glycoprotein. It has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. von Willebrand factor is synthesized by endothelial cells and stored in the Weibel-Palade granules. It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII. von Willebrand factor is one of the most useful markers to identify endothelial (or megakaryocytic) lineage of neoplasms. As not all endothelial cells synthesize/ store) this molecule, about 30% of tumors of vascular origin fail to stain for factor VIII related antigen, regardless of whether they are benign or malignant. Staining for factor VIII related antigen has also been used to measure angiogenesis, an indicator of tumor recurrence.

Specifications

• Accession Number: P04275
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: F8VWF,VWD
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Sensitivity: 0.13 ng/mL
• Conjugate: Biotin
• Protein Family: Other Proteins
• Specificity: This ELISA antibody pair detects human vWF. Other species not determined.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 7450
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Angiogenesis
• Synonym: F8VWF, VWD
• Test Time: 1 hr. 20 min.