Invitrogen™ Vitronectin (VTN) Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHVTN

Product Code. 15465173

Description

The Human Vitronectin (VTN) ELISA quantitates Hu VTN in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VTN. Principle of the method The Human VTN solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75 kDa and 65 kDa forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognises fibronectin.

Specifications

• Accession Number: P04004
• Assay Sensitivity: 0.8 ng/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 7448
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Cardiovascular Research
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 0.819-200 ng/mL
• Calibration Range: 0.8ng/mL to 200ng/mL
• Conjugate: Biotin
• Protein Family: Other Proteins
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human A2M, ApoA1, ApoC1, ApoC2, ApoC3, ApoE, ApoH, Artemin, BMP-6, Calbindin D, Ck beta 8-1 , CNTF, EMMPRIN, Endostatin, Fibrinogen, FLRG, GITR Ligand, GPBB, Hemoglobin, HTRA2, IL-17E, IL-1R3, Lactoferrin, Mesothelin, PAPP-A, PDECGF, PDGF Ralpha, Pepsinogen II, Periostin, Persephin, PTH, RAP, SAA, Serpin F1, Tie-1, tPA, TSP-2, Ubiquitin+1, Vitronectin, vWF
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: V75,VN,VNT
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 2 μL; Serum, 2 μL; Supernatant, 100 μL
• Synonym: V75, VN, VNT
• Test Time: 1 hr. 20 min.