Invitrogen™ VISTA Monoclonal Antibody (MIH64), Brilliant Ultra Violet™ 737, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Invitrogen™ 367-1083-80

Product Code. 17423382

Description

This MIH64 monoclonal antibody recognizes mouse VISTA also known as B7-H5 or PD-H1. This MIH64 antibody will work in flow cytometry on both native and paraformaldehyde-fixed cells. Applications Reported: This MIH64 antibody has been reported for use in flow cytometric analysis. Applications Tested: This MIH64 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1.0 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Brilliant Ultraviolet 737 is a tandem dye that emits at 732 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser.

VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene. Although VISTA bears sequence homology to PD-L1 it has a different expression pattern. In both mouse and humans, VISTA is expressed predominantly on hematopoietic cells with the highest densities on myeloid cells, and lower levels on T cells. It is usually absent on B cells. VISTA expressed by antigen presenting cells has been shown to directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it a target for cancer immunotherapy. The reported ligands of VISTA include CD28H, VSIG-3 and VSIG-8.

Specifications

• Antigen: VISTA
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9D659
• Gene Symbols: VSIR
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 74048
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: MIH64
• Conjugate: Brilliant Ultraviolet 737
• Gene: VSIR
• Gene Alias: 4632428N05Rik; B7H5; B7-H5; C10orf54; C1H10orf54; C28H10orf54; C4H10orf54; chromosome 10 open reading frame 54; DD1alpha; Death Domain1alpha; Dies1; differentiation of ESCs 1; GI24; PD-1H; PDCD1 homolog; PD-H1; platelet receptor Gi24; PP2135; RIKEN cDNA 4632428N05 gene; SISP1; sisp-1; Stress-induced secreted protein-1; UNQ730/PRO1412; V-domain Ig suppressor of T cell activation; VISTA; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; VSIR; V-type immunoglobulin domain-containing suppressor of T-cell activation
• Host Species: Rat
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ