Invitrogen™ Vinculin Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA580216

Product Code. 15995775

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: Rat Heart Tissue, Rat Brain Tissue, Rat Liver Tissue, U87 Cell, SMMC Cell, HEPA Cell, HELA Cell, HT1080 Cell. IHC: Human Mammary Cancer Tissue, Rat Intestine Tissue.

Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.

Specifications

• Antigen: Vinculin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: VCL
• Gene Alias: 9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; epididymis luminal protein 114; HEL114; metavinculin; meta-vinculin; metavinculin {ECO:0000250; MV; MVCL; RP11-178G16.3; UniProtKB:P18206}; unnamed protein product; Vcl; vcl {ECO:0000250; VIN; vinc; vinc 1; VINC1; Vinculin; vinculin {ECO:0000250
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 22330, 7414
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 500 μg/mL
• Formulation: PBS with 5mg BSA and 0.05mg sodium azide, 0.05mg thimerosal
• Gene Accession No.: P18206, Q64727
• Gene Symbols: VCL
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Vinculin (173-188aa KMIDERQQELTHQEHR).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG